Moleculaire klonering en karakterisering van de genpromotor met hoge affiniteit van de kaliumtransporter (AlHKT2;1) van halofyt Aeluropus lagopoides
HKT-subfamilie II functioneert als Na + – Okay + co-transporter en voorkomt dat planten zoutstress krijgen. Een promotorgebied van 760 bp van AlHKT2;1 werd geïsoleerd, gesequenced en gekloneerd. De promotor D1 van volledige lengte heeft veel cis-regulerende elementen zoals MYB, MBS, W field, ABRE enz. die betrokken zijn bij abiotische stressreacties. D1 en daaropvolgende 5′-deleties werden gekloneerd in pCAMBIA1301 en onderzocht op de werkzaamheid ervan bij stressomstandigheden in heteroloog systeem. Blauwe kleur kleuring werd waargenomen in bloemblaadjes, helmknop kwab en openspringen spleet van anther in T 0 planten. De T 1 zaailing toonde kleuring in nerven, shoot vaatstelsel en wortel, behalve wortel tip. T 1 zaailingen werden onderworpen aan NaCl,KCl en NaCl + KCl en ABA- spanningen. GUS-activiteit werd gekwantificeerd met een 4-methylumbelliferylglucuronide (4-MUG)-assay onder controle- en stressomstandigheden.
De kleinste deletie-D4 vertoonde ook GUS-expressie, maar de hoogste activiteit werd waargenomen in D2 in vergelijking met de promotor van volledige lengte en andere deleties. De elektroforetische mobiliteitsverschuivingstest met behulp van stress-geïnduceerd eiwit met verschillende promotordeleties onthulde een prominentere binding in D2 . Deze resultaten suggereren dat AlHKT2;1-promotor betrokken is bij abiotische stressrespons en dat deletie D2 voldoende zou kunnen zijn om de stress-induceerbare expressie aan te sturen van verschillende genen die betrokken zijn bij het bieden van stresstolerantie in planten
cDNA- klonering , expressie en antischimmelactiviteit van chitinase van Ficus microcarpa-latex: verschil in antischimmelwerking van chitinase met en zonder chitinebindend domein
Een chitine-bindend domein zou kunnen bijdragen aan het antischimmelvermogen van chitinase door zijn affiniteit voor de laterale wand van de schimmel door hydrofobe interacties. Complementair DNA dat codeert voor het antischimmelchitinase van gazyumaru (Ficus microcarpa), aangeduid als GlxChiB, werd gekloond en tot expressie gebracht in Escherichia coli-cellen. De resultaten van cDNA-klonering toonden aan dat de voorloper van GlxChiB een N-terminaal endoplasmatisch reticulum-targetingsignaal en C-terminaal vacuolair targetingsignaal heeft, terwijl rijpe GlxChiB is samengesteld uit een N-terminaal koolhydraatbindend modulefamilie -18-domein (CBM18)en een C-terminaal glycoside-hydrolase-familie-19-domein (GH19) met een korte linker. Om de rol van het CBM18-domein in de antischimmelactiviteit van chitinase te verduidelijken, werden het recombinante GlxChiB (wildtype) en het katalytische domein (CatD) ervan gebruikt in kwantitatieve antischimmeltesten onder verschillende ionsterkten en microscopische waarnemingen tegen de schimmel Trichoderma viride.
De antischimmelactiviteit van het wildtype was sterker dan die van CatD onder alle ionsterkte- omstandigheden die in deze take a look at werden gebruikt; de antischimmelactiviteit van CatD werd echter zwakker met toenemende ionsterkte, terwijl die van het wildtype behouden bleef. De resultaten bij hoge ionsterkte bevestigden verder de bijdrage van het CBM18-domein aan het antischimmelvermogen van GlxChiB. De microscopische waarnemingen toonden duidelijk aan dat het wildtype inwerkte op zowel de uiteinden als de zijwand van schimmelhyfen , terwijl CatD alleen op de uiteinden inwerkte. Deze resultaten suggereren dat het CBM18-domein zou kunnen bijdragen aan het antischimmelvermogen van chitinase door zijn affiniteit met de laterale wand van de schimmel door hydrofobe interacties
Toepassing van de aangepaste handgemaakte kloontechniek op varkens
Hoewel somatische celkernoverdracht (SCNT) vaak wordt gebruikt om gekloonde dieren in laboratoria te produceren, is deze techniek duur en inefficiënt. Daarom is de handgemaakte kloontechniek (HMC) voorgesteld om het kloonproces te vereenvoudigen en te bevorderen, maar HMC verspilt veel eicellen en leidt tot mitochondriale heteroplasmie. Om deze problemen op te lossen, stellen we een gemodificeerde, handgemaakte kloontechniek (mHMC) voor die gebruik maakt van eenvoudige laboratoriumapparatuur, dwz een Pasteur-pipet en een alcohollamp, om deze toe te passen op het klonen van varkensembryo’s. Om de toepassing van mHMC op het klonen van varkens te valideren , worden embryo’s geproduceerd through SCNT en mHMC vergeleken met behulp van meerdere methoden, zoals enucleatie-efficiëntie, oxidatieve stress, embryo-ontwikkelingscompetentie en genexpressie.
De resultaten tonen geen significante verschillen tussen technieken, behalve in de enucleatie-efficiëntie. De 8-cel en 16-cel embryonale ontwikkelingscompetentie en Oct4-expressieniveaus vertonen significante verschillen. De snelheid van blastocysten is echter niet important verschillend tussen mHMC en SCNT . Deze studie verifieert dat gekloonde embryo’s die zijn afgeleid van de twee technieken vergelijkbare generatie- en ontwikkelingscompetentie vertonen. We stellen dus voor dat mHMC SCNT zou kunnen vervangen voor eenvoudiger en goedkoper klonen van varkens.
Description: Human secreted CD28-Fc fusion protein, also known as TP44, GenBank Accession No. NM_006139, a.a 19-152, expressed in a HEK293 cell expression system. MW = 41.4 kDa (monomer). This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
Description: Human herpesvirus entry mediator A, also known as HVEM, TNFRSF14, CD270, and HVEA, GenBank Accession No. NM_003820, a.a. 37-202 fused to human IgG1 Fc, expressed in a HEK293 cell expression system. MW = 44.2 kDa. This protein runs at a higher M.W. by SDSPAGE due to glycosylation.
Description: Human secreted CD40, Fc fusion protein, also known as Tumor Necrosis Factor Receptor Superfamily Member 5, TNFRSF5, and Bp50, GenBank Accession No. NM_001250, a.a. 21-193 expressed in a HEK293 cell expression system. MW = 45.9 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human secreted CD27, Fc fusion protein, also known as Tumor Necrosis Factor Receptor Superfamily Member 7, TNFRSF7, and T14, GenBank Accession No. NM_001242, a.a. 21-192 expressed in a HEK293 cell expression system. MW = 45.8 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human CD47, Fc fusion protein, also known as leukocyte surface antigen CD47, Rh-related antigen, integrin-associated protein, antigenic surface determinant protein OA3, antigen identified by monoclonal antibody 1D8, IAP, and MER6. GenBank Accession No. NM_001777, a.a. 19-139 expressed in a HEK293 cell expression system. MW = 40 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human T-cell immunoreceptor with Ig and_x000D_ITIM domains (TIGIT), also known as V-set_x000D_and immunoglobulin domain-containing_x000D_protein 9, VSIG9, V-set and transmembrane_x000D_domain-containing protein 3, and VSTM3,_x000D_GenBank Accession No. NM_173799, a.a._x000D_22-141 fused to Fc region of human IgG,_x000D_expressed in a HEK293 cell expression_x000D_system. MW = 39.7 kDa. This protein runs_x000D_at a higher MW by SDS-PAGE due to_x000D_glycosylation.
Description: Human CD44, also known as epican, extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, or phagocytic glyco-protein I (PGP-1), GenBank Accession No. NM_000610, a.a. 21-220, fused with Fc region of human IgG, expressed in a HEK293 cell expression system. MW = 48.7 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human secreted lymphotoxin beta receptor_x000D_(LTBR)-Fc fusion protein, also known as_x000D_Tumor Necrosis Factor C Receptor_x000D_(TNFCR), and CD18, GenBank Accession_x000D_No. NM_002342, a.a 28-219, expressed in_x000D_a HEK293 cell expression system. MW = 48_x000D_kDa (monomer). This protein runs at a_x000D_higher apparent M.W. by SDS-PAGE due to_x000D_glycosylation.
Description: Mouse secreted lymphotoxin beta receptor_x000D_(mLTBR)-Fc fusion protein, also known as,_x000D_Tumor Necrosis Factor C Receptor_x000D_(TNFCR), and CD18, GenBank Accession_x000D_No. NM_010736, a.a 28-221, expressed in_x000D_a HEK293 cell expression system. MW =_x000D_48.8 kDa (monomer). Endotoxin level_x000D_<0.001 EU/ug.This protein runs at a higher_x000D_apparent M.W. by SDS-PAGE due to_x000D_glycosylation.
Description: Secreted frizzled receptor (FZD1)-Fc fusion_x000D_protein and WNT receptor, also known as_x000D_FZD1, GenBank Accession No.BC_051271,_x000D_a.a. 73-253, expressed in a HEK293 cell_x000D_expression system. MW = 46.4 kDa_x000D_(monomer). This protein runs at a higher_x000D_apparent M.W. by SDS-PAGE due to_x000D_glycosylation.
Description: Secreted frizzled receptor (FZD10)-Fc_x000D_fusion protein and WNT receptor, also_x000D_known as FZD10, GenBank Accession No._x000D_BC_074997, a.a. 21-161, expressed in a_x000D_HEK293 cell expression system. MW = 42.7_x000D_kDa (monomer). This protein runs at a_x000D_higher apparent M.W. by SDS-PAGE due to_x000D_glycosylation.
Description: Secreted frizzled receptor (FZD4)-Fc fusion_x000D_protein and WNT receptor, also known as_x000D_FZD4, GenBank Accession No._x000D_BC_114527, a.a. 37-180, expressed in a_x000D_HEK293 cell expression system. MW = 42.9_x000D_kDa (monomer). This protein runs at a_x000D_higher apparent M.W. by SDS-PAGE due to_x000D_glycosylation.
Description: Secreted frizzled receptor (FZD7)-Fc fusion_x000D_protein and WNT receptor, also known as_x000D_FZD7, GenBank Accession No._x000D_BC_015915, a.a. 33-185, expressed in a_x000D_HEK293 cell expression system. MW = 43.3_x000D_kDa (monomer). This protein runs at a_x000D_higher apparent M.W. by SDS-PAGE due to_x000D_glycosylation.
Description: Human B- and T-lymphocyte attenuator (BTLA)-Fc fusion protein, also known as CD272, GenBank Accession No. NM_181780, a.a. 31-150, expressed in a HEK293 cell expression system. MW = 40.3 kDa (monomer). This protein runs at a higher apparent M.W. by SDS-PAGE due to glycosylation.
Description: Human secreted LAG3, Fc fusion protein, also known as Lymphocyte-Activation Gene 3 and CD223. GenBank Accession No. NM_002286, a.a. 23-450 expressed in a HEK293 cell expression system. MW = 73.1 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human secreted CTLA4 , Fc fusion protein, also known as Cytotoxic T-lymphocyte-associated protein 4 and CD152. GenBank Accession No. NM_005214, a.a. 36-162 expressed in a HEK293 cell expression system. MW = 40.3 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human secreted TIM-3, Fc fusion protein, also known as T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, T-cell and immunoglobulin and mucin domain-containing protein 3, TIMD-3, Hepatitis A virus cellular receptor 2, and HAVCR-2. GenBank Accession No. NM_032782.4, a.a. 22-200 expressed in a HEK293 cell expression system. MW = 46.5 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human secreted GITR, Fc fusion protein, also known as Glucocorticoid-induced TNFR-Related Protein, Tumor Necrosis Factor Receptor Superfamily member 18, TNFRSF18, Activation-Inducible TNFR Family Receptor, AITR, and CD357, GenBank Accession No. NM_004195, a.a. 26-161 expressed in a HEK293 cell expression system. MW = 41.2 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human secreted OX40, Fc fusion protein,_x000D_also known as Tumor Necrosis Factor_x000D_Receptor Superfamily Member 4,_x000D_TNFRSF4, and CD134, GenBank_x000D_Accession No. NM_003327, a.a. 29-216_x000D_expressed in a HEK293 cell expression_x000D_system. MW = 46.8 kDa (monomer). This_x000D_protein runs at a higher MW by SDS-PAGE_x000D_due to glycosylation.
Description: Human ICOS, Fc fusion protein, also known as inducible T-cell costimulator and CD antigen 278, activation-inducible lymphocyte immunomediatory molecule, AILIM, CD278, and CVID1. GenBank Accession No. NM_012092, a.a. 21-140 expressed in a HEK293 cell expression system. MW = 40.2 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human CD226 antigen also_x000D_known as DNAX accessory molecule 1_x000D_(DNAM-1), GenBank Accession No._x000D_NM_006566, a.a. 19-247 fused to Fc region_x000D_of human IgG1, expressed in a HEK293 cell_x000D_expression system. MW = 52.8 kDa_x000D_(monomer). This protein runs at a higher_x000D_MW by SDS-PAGE due to glycosylation.
Description: Rhesus monkey angiotensin I converting enzyme 2 (ACE2), also known as ACEH, Genbank Accession No.: ACI04553.1, a.a. 18-739, fused at the C-terminus of the Fc portion of human IgG1, expressed in a HEK293 expression system, MW= 119 kDa. This protein runs at a higher MW due to glycosylation.
Description: Rhesus monkey angiotensin I converting enzyme 2 (ACE2), also known as ACEH, Genbank Accession No.: ACI04553.1, a.a. 18-739, fused at the C-terminus of the Fc portion of human IgG1, expressed in a HEK293 expression system, MW= 119 kDa. This protein runs at a higher MW due to glycosylation.
Description: Human IL-6 Receptor, Genbank # NM_000565, a.a. 20-365 with C-terminal fusion to human IgG1 Fc region, expressed in HEK293 cells. MW = 65 kDa + glycans.
Description: Human IL-6 Receptor, Genbank # NM_000565, a.a. 20-365 with C-terminal fusion to human IgG1 Fc region, expressed in HEK293 cells. MW = 65 kDa + glycans.
Description: A sandwich quantitative ELISA assay kit for detection of Human Fusion (FUS) in samples from tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Fusion (FUS) in samples from tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Fusion (FUS) in samples from tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Fusion (FUS) in samples from tissue homogenates or other biological fluids.
Description: Pre-made lentiviral particles expressing a CFP-LC3 fusion target (NM_022818.4), provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Description: Pre-made lentiviral particles expressing a GFP-LC3 fusion target (NM_022818.4), provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Description: Pre-made lentiviral particles expressing a RFP-LC3 fusion target (NM_022818.4), provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Description: Pre-made lentiviral particles expressing (CFP-human Annexin 5) fusion contruct under suCMV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Description: Pre-made lentiviral particles expressing (GFP-human Annexin 5) fusion contruct under suCMV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Description: Pre-made lentiviral particles expressing (RFP-human Annexin 5) fusion contruct under suCMV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Description: Pre-made lentiviral particles expressing (CFP-human Actin) fusion contruct under suCMV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Description: Pre-made lentiviral particles expressing (GFP-human Actin) fusion contruct under suCMV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Description: Pre-made lentiviral particles expressing (RFP-human Actin) fusion contruct under suCMV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Description: Pre-made lentiviral particles expressing (CFP-HIV-1 Tat) fusion contruct under suCMV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Description: Pre-made lentiviral particles expressing (GFP-HIV-1 Tat) fusion contruct under suCMV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Description: Pre-made lentiviral particles expressing (RFP-HIV-1 Tat) fusion contruct under suCMV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Description: Pre-made lentiviral particles expressing (CFP-human P53) fusion contruct under suCMV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Description: Pre-made lentiviral particles expressing (GFP-human P53) fusion contruct under suCMV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Description: Pre-made lentiviral particles expressing (RFP-human P53) fusion contruct under suCMV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Description: Pre-made lentiviral particles expressing (CFP-human Zyxin) fusion contruct under suCMV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Description: Pre-made lentiviral particles expressing (GFP-human Zyxin) fusion contruct under suCMV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Description: Pre-made lentiviral particles expressing (RFP-human Zyxin) fusion contruct under suCMV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Description: Pre-made lentiviral particles expressing afusion target of (CFP-human KCNN4), provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Description: Pre-made lentiviral particles expressing afusion target of (RFP-human KCNN4), provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Description: Pre-made lentiviral particles expressing afusion target of (CFP-human CLCN2), provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Description: Pre-made lentiviral particles expressing afusion target of (RFP-human CLCN2), provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Description: Pre-made lentiviral particles expressing afusion target of (CFP-human TRPV1), provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Description: Pre-made lentiviral particles expressing afusion target of (RFP-human TRPV1), provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Description: Pre-made lentiviral particles expressing afusion target of (CFP-human TRPC3), provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Description: Pre-made lentiviral particles expressing afusion target of (RFP-human TRPC3), provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Description: Pre-made lentiviral particles expressing a fusion target of (CFP-human CSF1) containing a Puromycin marker, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Description: Pre-made lentiviral particles expressing a fusion target of (GFP-human CSF1) containing a Puromycin marker, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Description: Pre-made lentiviral particles expressing a fusion target of (RFP-human CSF1) containing a Puromycin marker, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Fusion (FUS) in tissue homogenates, cell lysates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Fusion (FUS) in tissue homogenates, cell lysates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Fusion (FUS) in tissue homogenates, cell lysates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Fusion (FUS) in tissue homogenates, cell lysates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Fusion (FUS) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Fusion (FUS) in Tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Fusion (FUS) in Tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Fusion (FUS) in Tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Fusion (FUS) in Tissue homogenates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Fusion (FUS) in samples from Tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.
Description: Human secreted B7-1-Fc fusion protein, also known as CD80. GenBank Accession No. NM_005191, a.a. 35-242 expressed in a HEK293 cell expression system. MW = 50.5 kDa (monomer). This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
Description: Human secreted CD44, also known as epican, extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, or phagocytic glycoprotein I (PGP-1), GenBank Accession No. NM_000610, a.a. 21-220 expressed in a HEK293 cell expression system, fused at the C-terminus to the Fc portion of human IgG1, and enzymatically biotin-labeled using Avi-tag™ technology. MW = 50.6 kDa (monomer). This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
Description: Human herpesvirus entry mediator A, also known as HVEM, TNFRSF14, CD270, and HVEA, GenBank Accession No. NM_003820, a.a. 37-202 fused to human IgG1 Fc, with C-terminal Avi-Tag™, expressed in a HEK293 cell expression system. MW = 46 kDa. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
Description: Human secreted B7-H5, Fc fusion protein, also known as Gi24, Dies1, VISTA, and SISP1, GenBank Accession No. NM_022153, a.a. 33-194 expressed in a HEK293 cell expression system. MW = 46.7 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human secreted CTLA4 , Fc fusion protein, also known as Cytotoxic T-lymphocyte-associated protein 4 and CD152. GenBank Accession No. NM_005214, a.a. 36-162 expressed in a HEK293 cell expression system. MW = 40.3 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human secreted B7-2, Fc fusion protein, also known as T-lymphocyte activation antigen CD86, B7.2, FUN-1, B70, BU63, and CD86. GenBank Accession No. NM_006889, a.a. 20-239 expressed in a HEK293 cell expression system. MW = 51.9 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human CD47, also known as leukocyte surface antigen CD47, Rh-related antigen, integrin-associated protein (IAP), antigenic surface determinant protein OA3, and MER6. GenBank Accession No. NM_001777, a.a. 19-139 expressed in a HEK293 cell expression system, fused at the C-terminus to the Fc portion of human IgG1, and enzymatically biotin-labeled using Avi-tag™ technology. MW = 42.2 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human CD47, also known as leukocyte surface antigen CD47, Rh-related antigen, integrin-associated protein (IAP), antigenic surface determinant protein OA3, and MER6. GenBank Accession No. NM_001777, a.a. 19-139 expressed in a HEK293 cell expression system, fused at the C-terminus to the Fc portion of human IgG1, and enzymatically biotin-labeled using Avi-tag™ technology. MW = 42.2 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human CD28 homolog (CD28H), also known as Transmembrane and immunoglobulin domain-containing protein 2 (TMIGD2) and immunoglobulin and prolinerich receptor 1 (IGPR-1). GenBank Accession No. NM_144615, a.a. 23-128 fused at the C-terminus to the Fc portion of human IgG1 followed by a C-terminal AviTag™ expressed in a HEK293 expression system. MW = 40 kDa. This protein is enzymatically biotinylated using Avi-tag™ technology. Biotinylation is confirmed to be greater than 90%. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human CD28 homolog (CD28H), also known as Transmembrane and immunoglobulin domain-containing protein 2 (TMIGD2) and immunoglobulin and prolinerich receptor 1 (IGPR-1). GenBank Accession No. NM_144615, a.a. 23-128 fused at the C-terminus to the Fc portion of human IgG1 followed by a C-terminal AviTag™ expressed in a HEK293 expression system. MW = 40 kDa. This protein is enzymatically biotinylated using Avi-tag™ technology. Biotinylation is confirmed to be greater than 90%. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Mouse secreted CD137, also known as_x000D_Tumor Necrosis Factor Receptor_x000D_Superfamily member 9, TNFRSF9, and ILA,_x000D_GenBank Accession No. NM_011612, a.a._x000D_24-187 fused at the C-terminus to the Fc_x000D_portion of human IgG1, expressed in a_x000D_HEK293 cell expression system. MW = 44_x000D_kDa (monomer). This protein runs at a_x000D_higher MW by SDS-PAGE due to_x000D_glycosylation.
Description: Human T-cell immunoreceptor_x000D_with Ig and ITIM domains (TIGIT), also_x000D_known as V-set and immunoglobulin_x000D_domain-containing protein 9 (VSIG9), and_x000D_V-set and transmembrane domaincontaining_x000D_protein 3 (VSTM3), GenBank_x000D_Accession No. NM_173799, a.a. 22-141_x000D_fused to Fc region of human IgG1 and_x000D_enzymatically biotinylated using AviTag™_x000D_technology, expressed in a HEK293 cell_x000D_expression system. MW = 41.6 kDa_x000D_(monomer). This protein runs at a higher_x000D_MW by SDS-PAGE due to glycosylation.
Description: Human T-cell immunoreceptor_x000D_with Ig and ITIM domains (TIGIT), also_x000D_known as V-set and immunoglobulin_x000D_domain-containing protein 9 (VSIG9), and_x000D_V-set and transmembrane domaincontaining_x000D_protein 3 (VSTM3), GenBank_x000D_Accession No. NM_173799, a.a. 22-141_x000D_fused to Fc region of human IgG1 and_x000D_enzymatically biotinylated using AviTag™_x000D_technology, expressed in a HEK293 cell_x000D_expression system. MW = 41.6 kDa_x000D_(monomer). This protein runs at a higher_x000D_MW by SDS-PAGE due to glycosylation.
Description: Human secreted CD58-Fc fusion protein, also known as LFA-3, GenBank Accession No. NM_001779, a.a. 29-215, fused at the C-terminus to the Fc portion of human IgG1, with C-terminal Avi-tag, expressed in a HEK293 cell expression system and enzymatically biotin-labeled using Avi-tag technology. MW = 50 kDa (monomer). This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
Description: Human secreted CD2-Fc fusion protein, also known as LFA-2, GenBank Accession No. NM_001767, a.a. 25-209, fused at the C-terminus to the Fc portion of human IgG1, with C-terminal Avi-tag, expressed in a HEK293 cell expression system and enzymatically biotin-labeled using Avi-tag technology. MW = 50 kDa (monomer). This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
Description: Human secreted CD2-Fc fusion protein, also known as LFA-2, GenBank Accession No. NM_001767, a.a. 25-209, fused at the C-terminus to the Fc portion of human IgG1, with C-terminal Avi-tag, expressed in a HEK293 cell expression system and enzymatically biotin-labeled using Avi-tag technology. MW = 50 kDa (monomer). This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
Description: Human KIR2DL1, also known as Killer cell immunoglobulin-like receptor 2DL1, CD158A, or NKAT-1, .GenBank Accession No. NM_014218, a.a. 22-245 fused at the C terminus to the Fc portion of human IgG1 followed by a C-terminal Avi-tag™ and expressed in a HEK293 cell expression system. MW=52 kDa. This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation is confirmed to be ≥90%. This protein runs at a higher MW due to glycosylation.
Description: Human KIR2DL2, also known as Killer cell immunoglobulin-like receptor 2DL2, CD158K, or NKAT-4, GenBank Accession No. NM_014219, a.a. 22-245 fused at the C terminus to the Fc portion of human IgG1 followed by a C-terminal Avi-tag™ and expressed in a HEK293 cell expression system. MW=53 kDa. This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation is confirmed to be ≥90%. This protein runs at a higher MW due to glycosylation.
Description: Human KIR2DL3, also known as Killer cell immunoglobulin-like receptor 2DL3, KIR2DS5 and KIRCL23. GenBank Accession No. NM_015868. a.a. 22-245 fused at the C terminus to the Fc portion of human IgG1 followed by a C-termina Avi-tag™ and expressed in a HEK293 cell expression system. MW=53 kDa This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation is confirmed to be ≥90%. This protein runs at a higher MW due to glycosylation.
Description: Human KIR2DL3, also known as Killer cell immunoglobulin-like receptor 2DL3, KIR2DS5 and KIRCL23. GenBank Accession No. NM_015868. a.a. 22-245 fused at the C terminus to the Fc portion of human IgG1 followed by a C-termina Avi-tag™ and expressed in a HEK293 cell expression system. MW=53 kDa This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation is confirmed to be ≥90%. This protein runs at a higher MW due to glycosylation.
Description: Human CD111, known as Poliovirus receptor-related 1, and nectin-1, GenBank Accession No. NM_002855, a.a. 31-355, with C-terminal Avi-Tag fused to the Fc portion of Human IgG1 and expressed in a HEK293 cell expression system. This protein runs at a higher MW by SDS-PAGE due to glycosylation. MW = 65 kDa.
Description: Woodchuck (Groundhog, Marmota monax) secreted programmed cell death 1 (PD-1) also known as, PDCD1, SLEB2, CD279 and HPD-L, GenBank Accession No. HQ403652.1, a.a 25-172, fused at the C-terminus to the Fc portion of Human IgG1, expressed in a HEK293 cell expression system. MW = 43 kDa. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
Description: Human B- and T-lymphocyte attenuator (BTLA), also known as CD272, with C-terminal Avi-TagTM fused to the Fc-region of Human IgG1. GenBank Accession No. NM_181780, a.a. 31-150, expressed in a HEK293 cell expression system. MW = 42 kDa (monomer). This protein runs at a higher apparent M.W. by SDS-PAGE due to glycosylation.
Description: Human secreted CD33, also known as Sialic Acid Binding Ig Like Lectin 3, Gp67 and Myeloid Cell Surface Antigen CD33, GenBank Accession No. NM_001772, a.a. 1-259, fused at the C-terminus of the Fc portion of human IgG1, with C-terminal Avi-tag™ , expressed in a HEK293 cell expression system. MW = 57 kDa.
Description: Human NKG2d, also known as, Killer Cell Lectin Like Receptor K1, KLRK1, and NK Cell Receptor D. GenBank Accession No. NM_007360.4, a.a. 38-216, with an N-terminal Avi-Tag™ fused to the Fc portion of human IgG1 and expressed in a HEK293 cell expression system. MW=53 kDa.
Description: Human MICA, also known as, MHC Class I Polypeptide-Related Sequence A, PERB11.1, and Stress Inducible Class I Homolog, GenBank Accession No. NM_001177519, a.a. 23-308, fused at the C-terminus to the Fc portion of human IgG1 followed by a C-terminal Avi-Tag™ and expressed in a HEK293 cell expression system. MW=62 kDa.
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Stamceltherapieën en voordelen van klonen van somatische celkernoverdracht in het COVID-19-tijdperk
Achtergrond: De wereldwijde noodsituatie op gezondheidsgebied van COVID-19 heeft de ontwikkeling van meerdere therapeutische modaliteiten noodzakelijk gemaakt, waaronder vaccinaties, antivirale middelen, ontstekingsremmende en cyto-immunotherapieën, enz. COVID-19-patiënten lijden aan schade aan verschillende organen en vasculaire structuren, dus vertonen ze meerdere gezondheidscrises. Mesenchymale stamcellen (MSC’s) zijn van belang voor de behandeling van acute respiratory misery syndrome (ARDS) veroorzaakt door SARS-CoV-2- infectie.
Hoofdgedeelte: Op stamcellen gebaseerde therapieën zijn geverifieerd op prospectieve voordelen in uitgebreide preklinische en klinische onderzoeken. MSC’s bieden potentiële voordelen voor de ontwikkeling van verschillende celtypen en organoïden voor het bestuderen van virus-menselijke interactie, het testen van geneesmiddelen, regeneratieve geneeskunde en immunomodulerende effecten bij COVID-19-patiënten. Behalve dat het de weg vrijmaakt om stamcelonderzoek en therapieën te verbeteren, biedt somatische celkernoverdracht (SCNT) unieke mogelijkheden voor een breed scala aan gezondheidstoepassingen, zoals patiëntspecifiekeof isogene cellen voor regeneratieve geneeskunde en het fokken van transgene dieren voor biomedische toepassingen. Omdat het een krachtig hulpmiddel is voor het herprogrammeren van het celgenoom, heeft de SCNT de bekendheid van recombinante therapieën en cellulaire geneeskunde vergroot in het huidige tijdperk van COVID-19. Omdat SCNT wordt gebruikt om patiëntspecifieke stamcellen te genereren, wordt afhankelijkheid van embryo’s voor het verkrijgen van stamcellen vermeden.
Conclusies: het klonen van nucleaire overdracht, dat een ideaal hulpmiddel is om gekloonde embryo’s te genereren, en de embryonale stamcellen zullen het testen van geneesmiddelen en cellulaire geneeskunde bij COVID-19 stimuleren.
Moleculair klonen van eend CD40 en zijn immuunfunctieonderzoek
Co-signaalmoleculen zijn celoppervlaktemoleculen die signalen naar andere cellen overbrengen om de immuunrespons positief (costimuleer) of negatief (cosuppress) te moduleren . Co-stimulerende signalen zijn sleutelfactoren bij het bepalen of T/B-cellen in staat zijn te reageren op specifieke antigenen en uiteindelijk een geschikte immuunrespons teweeg te brengen. In deze studie werd de cDNA-sequentie die het volledige coderende body van het co-stimulerende molecuul duck CD40-gen bevat, gekloneerd en voor de eerste keer gerapporteerd , en het gemedieerde antivirale aangeboren immuunsysteem ervan werd in vitro geverifieerd. Resultaten suggereerden dat eenden CD40- molecuul een belangrijke rol speelt in de aangeboren immuunrespons tegen sommige virussen. Deze gegevens zullen gunstig zijn voor het verdere begrip van het aviaire immuunsysteem.
Klonen en heterologe expressie van een nieuw xylanase-gen TAX1 van Trichoderma atroviride en de toepassing ervan bij de deconstructie van Corn Stover
Xylanase speelt een cruciale rol bij het efficiënte gebruik van xylan, dat tot 30% van de droge stof van de plant uitmaakt. De productiekosten van xylanase blijven echter hoog en de enzymatische eigenschappen van xylanasen van de meeste micro-organismen zijn niet geschikt voor industriële productie. Daarom is het van groot belang om nieuwe en efficiënte xylanasen te ontdekken en te ontwikkelen. In deze studie werd het xylanasegen TAX1 (672 bp cDNA) gekloneerd uit Trichoderma atroviride 3.3013 en tot expressie gebracht in Pichia pastoris. Het TAX1-gen codeerde voor een eiwit van 223-aminozuren (TAX1) met een molecuulgewicht van 24,2 kDa dat een hoge gelijkenis vertoonde met glycosidehydrolasefamilie 11. Enzymactiviteitstest bevestigde dat de recombinante xylanase TAX1 optimale activiteit had (215,Three IE/ml) bij 50 °C en pH 6,0.
Stabiele werkomstandigheden werden gemeten als pH 4,0-7,Zero en 40-60°C. Door Zn2 + toe te voegen , werd de relatieve enzymatische activiteit van recombinant TAX1 met 26% verhoogd . Het recombinante xylanase vertoonde een hoge activiteit tegen berkenhoutxylan en maïsstoof. De Okay m en ok cat van xylaan en maisstelen was 0,36 mg / ml en 0,204 S -1 en 0,48 mg / ml en 0,149 S -1 resp. De enzymatische activiteit van de TAX1 geproduceerd door P. pastoris was ongeveer 2,4-Four keer hoger dan die van direct geïsoleerd uit T. atroviride, dus gemanipuleerde P. pastoris voor xylanaseproductie zou een ideale kandidaat kunnen zijn voor industriële enzymproductie.
Cold Fusion Cloning Kit with Competent Cells (50 rxns) International Sales Only
Description: The Quick PCR™ Plus Assembly Kit is used as a molecular cloning tool to assemble long DNA fragment from multiple smaller fragments, or to insert DNA into a plasmid in a single reaction. The main kit component is a ready-to-use mix of enzymes in reaction buffer at a 2-fold concentration. This kit also includes chemically competent E. coli cells (another version of the kit does not include competent cells, BPS Bioscience #78531).
Description: The Quick PCR™ Plus Assembly Kit is used as a molecular cloning tool to assemble long DNA fragment from multiple smaller fragments, or to insert DNA into a plasmid in a single reaction. The main kit component is a ready-to-use mix of enzymes in reaction buffer at a 2-fold concentration. This kit also includes chemically competent E. coli cells (another version of the kit does not include competent cells, BPS Bioscience #78531).
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.
Description: An accurate, simple, fast (15 min) and inexpensive screening tool for the identification of protein putrefaction in the gastrointestinal tract. For research use only, not intended for diagnostic use. The Indican Reagent is corrosive. It is recommended to perform the test in a chemical fume hood. Wear gloves, goggles and protective clothing. Key Features: Convenient. Only need to pipette 2 mL urine into the ready reagent vial, mix and read the indican level from a color chart. Fast: 15 min. Method: Obermeyer (Improved). Samples: Urine. Species: Human. Procedure: Assay takes 15 min. Kit size: 20 tests.
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids. The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long. The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of the Monkeypox Virus DNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channel 530nm with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the 560nm fluorescence of the internal control (IC). An external positive control defined as 1×10^7 copies/ml is supplied which allow the determination of the gene load.
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids.The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long.The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of theMonkeypox VirusDNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channelFAM with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control defined as 1×107copies/ml is supplied which allow the determination of the gene load.
Description: BirA-transformed Competent E. coli cells are supplied as 10 x 50 µl vials._x000D_Strain BL21, a chemically competent E. coli B strain, contains an IPTG-inducible BirA_x000D_expression plasmid and constitutively-expressed streptomycin/spectinomycin resistance gene._x000D_These cells are compatible with most cloning vectors and are suitable for expression and_x000D_biotinylation of recombinant proteins using AviTag™ technology.
