Moleculaire klonering en karakterisering van de genpromotor met hoge affiniteit van de kaliumtransporter (AlHKT2;1) van halofyt Aeluropus lagopoides
HKT-subfamilie II functioneert als Na + – Okay + co-transporter en voorkomt dat planten zoutstress krijgen. Een promotorgebied van 760 bp van AlHKT2;1 werd geïsoleerd, gesequenced en gekloneerd. De promotor D1 van volledige lengte heeft veel cis-regulerende elementen zoals MYB, MBS, W field, ABRE enz. die betrokken zijn bij abiotische stressreacties. D1 en daaropvolgende 5′-deleties werden gekloneerd in pCAMBIA1301 en onderzocht op de werkzaamheid ervan bij stressomstandigheden in heteroloog systeem. Blauwe kleur kleuring werd waargenomen in bloemblaadjes, helmknop kwab en openspringen spleet van anther in T 0 planten. De T 1 zaailing toonde kleuring in nerven, shoot vaatstelsel en wortel, behalve wortel tip. T 1 zaailingen werden onderworpen aan NaCl,KCl en NaCl + KCl en ABA- spanningen. GUS-activiteit werd gekwantificeerd met een 4-methylumbelliferylglucuronide (4-MUG)-assay onder controle- en stressomstandigheden.
De kleinste deletie-D4 vertoonde ook GUS-expressie, maar de hoogste activiteit werd waargenomen in D2 in vergelijking met de promotor van volledige lengte en andere deleties. De elektroforetische mobiliteitsverschuivingstest met behulp van stress-geïnduceerd eiwit met verschillende promotordeleties onthulde een prominentere binding in D2 . Deze resultaten suggereren dat AlHKT2;1-promotor betrokken is bij abiotische stressrespons en dat deletie D2 voldoende zou kunnen zijn om de stress-induceerbare expressie aan te sturen van verschillende genen die betrokken zijn bij het bieden van stresstolerantie in planten
cDNA- klonering , expressie en antischimmelactiviteit van chitinase van Ficus microcarpa-latex: verschil in antischimmelwerking van chitinase met en zonder chitinebindend domein
Een chitine-bindend domein zou kunnen bijdragen aan het antischimmelvermogen van chitinase door zijn affiniteit voor de laterale wand van de schimmel door hydrofobe interacties. Complementair DNA dat codeert voor het antischimmelchitinase van gazyumaru (Ficus microcarpa), aangeduid als GlxChiB, werd gekloond en tot expressie gebracht in Escherichia coli-cellen. De resultaten van cDNA-klonering toonden aan dat de voorloper van GlxChiB een N-terminaal endoplasmatisch reticulum-targetingsignaal en C-terminaal vacuolair targetingsignaal heeft, terwijl rijpe GlxChiB is samengesteld uit een N-terminaal koolhydraatbindend modulefamilie -18-domein (CBM18)en een C-terminaal glycoside-hydrolase-familie-19-domein (GH19) met een korte linker. Om de rol van het CBM18-domein in de antischimmelactiviteit van chitinase te verduidelijken, werden het recombinante GlxChiB (wildtype) en het katalytische domein (CatD) ervan gebruikt in kwantitatieve antischimmeltesten onder verschillende ionsterkten en microscopische waarnemingen tegen de schimmel Trichoderma viride.
De antischimmelactiviteit van het wildtype was sterker dan die van CatD onder alle ionsterkte- omstandigheden die in deze take a look at werden gebruikt; de antischimmelactiviteit van CatD werd echter zwakker met toenemende ionsterkte, terwijl die van het wildtype behouden bleef. De resultaten bij hoge ionsterkte bevestigden verder de bijdrage van het CBM18-domein aan het antischimmelvermogen van GlxChiB. De microscopische waarnemingen toonden duidelijk aan dat het wildtype inwerkte op zowel de uiteinden als de zijwand van schimmelhyfen , terwijl CatD alleen op de uiteinden inwerkte. Deze resultaten suggereren dat het CBM18-domein zou kunnen bijdragen aan het antischimmelvermogen van chitinase door zijn affiniteit met de laterale wand van de schimmel door hydrofobe interacties
Toepassing van de aangepaste handgemaakte kloontechniek op varkens
Hoewel somatische celkernoverdracht (SCNT) vaak wordt gebruikt om gekloonde dieren in laboratoria te produceren, is deze techniek duur en inefficiënt. Daarom is de handgemaakte kloontechniek (HMC) voorgesteld om het kloonproces te vereenvoudigen en te bevorderen, maar HMC verspilt veel eicellen en leidt tot mitochondriale heteroplasmie. Om deze problemen op te lossen, stellen we een gemodificeerde, handgemaakte kloontechniek (mHMC) voor die gebruik maakt van eenvoudige laboratoriumapparatuur, dwz een Pasteur-pipet en een alcohollamp, om deze toe te passen op het klonen van varkensembryo’s. Om de toepassing van mHMC op het klonen van varkens te valideren , worden embryo’s geproduceerd through SCNT en mHMC vergeleken met behulp van meerdere methoden, zoals enucleatie-efficiëntie, oxidatieve stress, embryo-ontwikkelingscompetentie en genexpressie.
De resultaten tonen geen significante verschillen tussen technieken, behalve in de enucleatie-efficiëntie. De 8-cel en 16-cel embryonale ontwikkelingscompetentie en Oct4-expressieniveaus vertonen significante verschillen. De snelheid van blastocysten is echter niet important verschillend tussen mHMC en SCNT . Deze studie verifieert dat gekloonde embryo’s die zijn afgeleid van de twee technieken vergelijkbare generatie- en ontwikkelingscompetentie vertonen. We stellen dus voor dat mHMC SCNT zou kunnen vervangen voor eenvoudiger en goedkoper klonen van varkens.
Description: Human secreted CD28-Fc fusion protein, also known as TP44, GenBank Accession No. NM_006139, a.a 19-152, expressed in a HEK293 cell expression system. MW = 41.4 kDa (monomer). This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
Description: Human herpesvirus entry mediator A, also known as HVEM, TNFRSF14, CD270, and HVEA, GenBank Accession No. NM_003820, a.a. 37-202 fused to human IgG1 Fc, expressed in a HEK293 cell expression system. MW = 44.2 kDa. This protein runs at a higher M.W. by SDSPAGE due to glycosylation.
Description: Human secreted CD40, Fc fusion protein, also known as Tumor Necrosis Factor Receptor Superfamily Member 5, TNFRSF5, and Bp50, GenBank Accession No. NM_001250, a.a. 21-193 expressed in a HEK293 cell expression system. MW = 45.9 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human secreted CD27, Fc fusion protein, also known as Tumor Necrosis Factor Receptor Superfamily Member 7, TNFRSF7, and T14, GenBank Accession No. NM_001242, a.a. 21-192 expressed in a HEK293 cell expression system. MW = 45.8 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human CD47, Fc fusion protein, also known as leukocyte surface antigen CD47, Rh-related antigen, integrin-associated protein, antigenic surface determinant protein OA3, antigen identified by monoclonal antibody 1D8, IAP, and MER6. GenBank Accession No. NM_001777, a.a. 19-139 expressed in a HEK293 cell expression system. MW = 40 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human CD44, also known as epican, extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, or phagocytic glyco-protein I (PGP-1), GenBank Accession No. NM_000610, a.a. 21-220, fused with Fc region of human IgG, expressed in a HEK293 cell expression system. MW = 48.7 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human T-cell immunoreceptor with Ig and_x000D_ITIM domains (TIGIT), also known as V-set_x000D_and immunoglobulin domain-containing_x000D_protein 9, VSIG9, V-set and transmembrane_x000D_domain-containing protein 3, and VSTM3,_x000D_GenBank Accession No. NM_173799, a.a._x000D_22-141 fused to Fc region of human IgG,_x000D_expressed in a HEK293 cell expression_x000D_system. MW = 39.7 kDa. This protein runs_x000D_at a higher MW by SDS-PAGE due to_x000D_glycosylation.
Eppendorf Multiporator Helix Fusion Chamber For Cell Fusion - EACH
Description: Human secreted TIM-3, Fc fusion protein, also known as T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, T-cell and immunoglobulin and mucin domain-containing protein 3, TIMD-3, Hepatitis A virus cellular receptor 2, and HAVCR-2. GenBank Accession No. NM_032782.4, a.a. 22-200 expressed in a HEK293 cell expression system. MW = 46.5 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Fusion Inhibitory Peptide (Z-D-Phe-Phe-Gly-OH, FIP, Virus Replication Inhibitory Peptide) is a potent inhibitor of the virus replication, by inhibiting the membrane fusing activity of a viral glycoprotein[1].
Description: Human secreted lymphotoxin beta receptor_x000D_(LTBR)-Fc fusion protein, also known as_x000D_Tumor Necrosis Factor C Receptor_x000D_(TNFCR), and CD18, GenBank Accession_x000D_No. NM_002342, a.a 28-219, expressed in_x000D_a HEK293 cell expression system. MW = 48_x000D_kDa (monomer). This protein runs at a_x000D_higher apparent M.W. by SDS-PAGE due to_x000D_glycosylation.
Description: Mouse secreted lymphotoxin beta receptor_x000D_(mLTBR)-Fc fusion protein, also known as,_x000D_Tumor Necrosis Factor C Receptor_x000D_(TNFCR), and CD18, GenBank Accession_x000D_No. NM_010736, a.a 28-221, expressed in_x000D_a HEK293 cell expression system. MW =_x000D_48.8 kDa (monomer). Endotoxin level_x000D_<0.001 EU/ug.This protein runs at a higher_x000D_apparent M.W. by SDS-PAGE due to_x000D_glycosylation.
Description: Human B- and T-lymphocyte attenuator (BTLA)-Fc fusion protein, also known as CD272, GenBank Accession No. NM_181780, a.a. 31-150, expressed in a HEK293 cell expression system. MW = 40.3 kDa (monomer). This protein runs at a higher apparent M.W. by SDS-PAGE due to glycosylation.
Description: Human secreted LAG3, Fc fusion protein, also known as Lymphocyte-Activation Gene 3 and CD223. GenBank Accession No. NM_002286, a.a. 23-450 expressed in a HEK293 cell expression system. MW = 73.1 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human secreted GITR, Fc fusion protein, also known as Glucocorticoid-induced TNFR-Related Protein, Tumor Necrosis Factor Receptor Superfamily member 18, TNFRSF18, Activation-Inducible TNFR Family Receptor, AITR, and CD357, GenBank Accession No. NM_004195, a.a. 26-161 expressed in a HEK293 cell expression system. MW = 41.2 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human secreted OX40, Fc fusion protein,_x000D_also known as Tumor Necrosis Factor_x000D_Receptor Superfamily Member 4,_x000D_TNFRSF4, and CD134, GenBank_x000D_Accession No. NM_003327, a.a. 29-216_x000D_expressed in a HEK293 cell expression_x000D_system. MW = 46.8 kDa (monomer). This_x000D_protein runs at a higher MW by SDS-PAGE_x000D_due to glycosylation.
Description: Human ICOS, Fc fusion protein, also known as inducible T-cell costimulator and CD antigen 278, activation-inducible lymphocyte immunomediatory molecule, AILIM, CD278, and CVID1. GenBank Accession No. NM_012092, a.a. 21-140 expressed in a HEK293 cell expression system. MW = 40.2 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Rhesus monkey angiotensin I converting enzyme 2 (ACE2), also known as ACEH, Genbank Accession No.: ACI04553.1, a.a. 18-739, fused at the C-terminus of the Fc portion of human IgG1, expressed in a HEK293 expression system, MW= 119 kDa. This protein runs at a higher MW due to glycosylation.
Description: Rhesus monkey angiotensin I converting enzyme 2 (ACE2), also known as ACEH, Genbank Accession No.: ACI04553.1, a.a. 18-739, fused at the C-terminus of the Fc portion of human IgG1, expressed in a HEK293 expression system, MW= 119 kDa. This protein runs at a higher MW due to glycosylation.
Description: Human secreted CTLA4 , Fc fusion protein, also known as Cytotoxic T-lymphocyte-associated protein 4 and CD152. GenBank Accession No. NM_005214, a.a. 36-162 expressed in a HEK293 cell expression system. MW = 40.3 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human CD226 antigen also_x000D_known as DNAX accessory molecule 1_x000D_(DNAM-1), GenBank Accession No._x000D_NM_006566, a.a. 19-247 fused to Fc region_x000D_of human IgG1, expressed in a HEK293 cell_x000D_expression system. MW = 52.8 kDa_x000D_(monomer). This protein runs at a higher_x000D_MW by SDS-PAGE due to glycosylation.
Description: Secreted frizzled receptor (FZD1)-Fc fusion_x000D_protein and WNT receptor, also known as_x000D_FZD1, GenBank Accession No.BC_051271,_x000D_a.a. 73-253, expressed in a HEK293 cell_x000D_expression system. MW = 46.4 kDa_x000D_(monomer). This protein runs at a higher_x000D_apparent M.W. by SDS-PAGE due to_x000D_glycosylation.
Description: Secreted frizzled receptor (FZD4)-Fc fusion_x000D_protein and WNT receptor, also known as_x000D_FZD4, GenBank Accession No._x000D_BC_114527, a.a. 37-180, expressed in a_x000D_HEK293 cell expression system. MW = 42.9_x000D_kDa (monomer). This protein runs at a_x000D_higher apparent M.W. by SDS-PAGE due to_x000D_glycosylation.
Description: Secreted frizzled receptor (FZD7)-Fc fusion_x000D_protein and WNT receptor, also known as_x000D_FZD7, GenBank Accession No._x000D_BC_015915, a.a. 33-185, expressed in a_x000D_HEK293 cell expression system. MW = 43.3_x000D_kDa (monomer). This protein runs at a_x000D_higher apparent M.W. by SDS-PAGE due to_x000D_glycosylation.
Description: Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes . Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake. Acts as a lipid sensor that regulates mitochondrial fusion by regulating activation of the JNK pathway. When dietary levels of stearate (C18:0) are low, promotes activation of the JNK pathway, resulting in HUWE1-mediated ubiquitination and subsequent degradation of the mitofusin MFN2 and inhibition of mitochondrial fusion. When dietary levels of stearate (C18:0) are high, TFRC stearoylation inhibits activation of the JNK pathway and thus degradation of the mitofusin MFN2.
Description: Secreted frizzled receptor (FZD10)-Fc_x000D_fusion protein and WNT receptor, also_x000D_known as FZD10, GenBank Accession No._x000D_BC_074997, a.a. 21-161, expressed in a_x000D_HEK293 cell expression system. MW = 42.7_x000D_kDa (monomer). This protein runs at a_x000D_higher apparent M.W. by SDS-PAGE due to_x000D_glycosylation.
Description: The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is similar to other coronaviruses, and is comprised of four key structural proteins: S, the spike protein, E, the envelope protein, M, the membrane protein, and N, the nucleocapsid protein (2). Coronavirus spike proteins are class I fusion proteins and harbor an ectodomain, a transmembrane domain, and an intracellular tail. The highly glycosylated ectodomain projects from the viral envelope surface and facilitates attachment and fusion with the host cell plasma membrane. The ectodomain can be further subdivided into host receptor-binding domain (RBD) (S1) and membrane-fusion (S2) subunits, which are produced upon proteolysis by host proteases at S1/S2 and S2’ sites. S1 and S2 subunits remain associated after cleavage and assemble into crown-like homotrimers. In humans, both SARS-CoV and SARS-CoV-2 spike proteins utilize the angiotensin-converting enzyme 2 (ACE2) protein as a receptor for cellular entry. Spike protein subunits represent a key antigenic feature of coronavirus virions, and therefore represent an important target of vaccines, novel therapeutic antibodies, and small-molecule inhibitors.
Description: Angiotensin-converting enzyme 2 (ACE2) is also known as ACEH (ACE homolog), is an integral membrane protein with considerable homologous to ACE, which belongs to the peptidase M2 family. ACE2 is an exopeptidase that catalyses the conversion of angiotensin I to the nonapeptide angiotensin, or the conversion of angiotensin II to angiotensin 1-7. ACE2 may be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, ACE-2 serve as functional receptor for the spike glycoprotein of both coronaviruses. ACE2 is activated by chloride and fluoride, but not bromide and Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. ACE2 is active from pH 6 to 9, and the optimum pH is 6.5 in the presence of 1 M NaCl.
Description: Human secreted B7-2, Fc fusion protein, also known as T-lymphocyte activation antigen CD86, B7.2, FUN-1, B70, BU63, and CD86. GenBank Accession No. NM_006889, a.a. 20-239 expressed in a HEK293 cell expression system. MW = 51.9 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
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Stamceltherapieën en voordelen van klonen van somatische celkernoverdracht in het COVID-19-tijdperk
Achtergrond: De wereldwijde noodsituatie op gezondheidsgebied van COVID-19 heeft de ontwikkeling van meerdere therapeutische modaliteiten noodzakelijk gemaakt, waaronder vaccinaties, antivirale middelen, ontstekingsremmende en cyto-immunotherapieën, enz. COVID-19-patiënten lijden aan schade aan verschillende organen en vasculaire structuren, dus vertonen ze meerdere gezondheidscrises. Mesenchymale stamcellen (MSC’s) zijn van belang voor de behandeling van acute respiratory misery syndrome (ARDS) veroorzaakt door SARS-CoV-2- infectie.
Hoofdgedeelte: Op stamcellen gebaseerde therapieën zijn geverifieerd op prospectieve voordelen in uitgebreide preklinische en klinische onderzoeken. MSC’s bieden potentiële voordelen voor de ontwikkeling van verschillende celtypen en organoïden voor het bestuderen van virus-menselijke interactie, het testen van geneesmiddelen, regeneratieve geneeskunde en immunomodulerende effecten bij COVID-19-patiënten. Behalve dat het de weg vrijmaakt om stamcelonderzoek en therapieën te verbeteren, biedt somatische celkernoverdracht (SCNT) unieke mogelijkheden voor een breed scala aan gezondheidstoepassingen, zoals patiëntspecifiekeof isogene cellen voor regeneratieve geneeskunde en het fokken van transgene dieren voor biomedische toepassingen. Omdat het een krachtig hulpmiddel is voor het herprogrammeren van het celgenoom, heeft de SCNT de bekendheid van recombinante therapieën en cellulaire geneeskunde vergroot in het huidige tijdperk van COVID-19. Omdat SCNT wordt gebruikt om patiëntspecifieke stamcellen te genereren, wordt afhankelijkheid van embryo’s voor het verkrijgen van stamcellen vermeden.
Conclusies: het klonen van nucleaire overdracht, dat een ideaal hulpmiddel is om gekloonde embryo’s te genereren, en de embryonale stamcellen zullen het testen van geneesmiddelen en cellulaire geneeskunde bij COVID-19 stimuleren.
Moleculair klonen van eend CD40 en zijn immuunfunctieonderzoek
Co-signaalmoleculen zijn celoppervlaktemoleculen die signalen naar andere cellen overbrengen om de immuunrespons positief (costimuleer) of negatief (cosuppress) te moduleren . Co-stimulerende signalen zijn sleutelfactoren bij het bepalen of T/B-cellen in staat zijn te reageren op specifieke antigenen en uiteindelijk een geschikte immuunrespons teweeg te brengen. In deze studie werd de cDNA-sequentie die het volledige coderende body van het co-stimulerende molecuul duck CD40-gen bevat, gekloneerd en voor de eerste keer gerapporteerd , en het gemedieerde antivirale aangeboren immuunsysteem ervan werd in vitro geverifieerd. Resultaten suggereerden dat eenden CD40- molecuul een belangrijke rol speelt in de aangeboren immuunrespons tegen sommige virussen. Deze gegevens zullen gunstig zijn voor het verdere begrip van het aviaire immuunsysteem.
Klonen en heterologe expressie van een nieuw xylanase-gen TAX1 van Trichoderma atroviride en de toepassing ervan bij de deconstructie van Corn Stover
Xylanase speelt een cruciale rol bij het efficiënte gebruik van xylan, dat tot 30% van de droge stof van de plant uitmaakt. De productiekosten van xylanase blijven echter hoog en de enzymatische eigenschappen van xylanasen van de meeste micro-organismen zijn niet geschikt voor industriële productie. Daarom is het van groot belang om nieuwe en efficiënte xylanasen te ontdekken en te ontwikkelen. In deze studie werd het xylanasegen TAX1 (672 bp cDNA) gekloneerd uit Trichoderma atroviride 3.3013 en tot expressie gebracht in Pichia pastoris. Het TAX1-gen codeerde voor een eiwit van 223-aminozuren (TAX1) met een molecuulgewicht van 24,2 kDa dat een hoge gelijkenis vertoonde met glycosidehydrolasefamilie 11. Enzymactiviteitstest bevestigde dat de recombinante xylanase TAX1 optimale activiteit had (215,Three IE/ml) bij 50 °C en pH 6,0.
Stabiele werkomstandigheden werden gemeten als pH 4,0-7,Zero en 40-60°C. Door Zn2 + toe te voegen , werd de relatieve enzymatische activiteit van recombinant TAX1 met 26% verhoogd . Het recombinante xylanase vertoonde een hoge activiteit tegen berkenhoutxylan en maïsstoof. De Okay m en ok cat van xylaan en maisstelen was 0,36 mg / ml en 0,204 S -1 en 0,48 mg / ml en 0,149 S -1 resp. De enzymatische activiteit van de TAX1 geproduceerd door P. pastoris was ongeveer 2,4-Four keer hoger dan die van direct geïsoleerd uit T. atroviride, dus gemanipuleerde P. pastoris voor xylanaseproductie zou een ideale kandidaat kunnen zijn voor industriële enzymproductie.
Cold Fusion Cloning Kit with Competent Cells (50 rxns) International Sales Only
Description: The Quick PCR™ Plus Assembly Kit is used as a molecular cloning tool to assemble long DNA fragment from multiple smaller fragments, or to insert DNA into a plasmid in a single reaction. The main kit component is a ready-to-use mix of enzymes in reaction buffer at a 2-fold concentration. This kit also includes chemically competent E. coli cells (another version of the kit does not include competent cells, BPS Bioscience #78531).
Description: The Quick PCR™ Plus Assembly Kit is used as a molecular cloning tool to assemble long DNA fragment from multiple smaller fragments, or to insert DNA into a plasmid in a single reaction. The main kit component is a ready-to-use mix of enzymes in reaction buffer at a 2-fold concentration. This kit also includes chemically competent E. coli cells (another version of the kit does not include competent cells, BPS Bioscience #78531).
Fast and Efficient Mutagenesis Kit without Competent Cells
Description: ProClone™ Competent Cells are high-efficiency, chemically competent DH5α (E. coli) cells that are optimized for use with abm’s versatile range of expression vectors. Transformation efficiency greater than 1x10^6 cfu/µg can be achieved using abm’s expression vectors, which are typically 3 to 4-fold larger in size and contain more complex genetic elements than the standard pUC19 plasmid. Reduced recombination and highly transformation efficiency make ProClone™ Competent Cells the premier choice for both routine and challenging subcloning projects.
Description: Applications: K599 Chemically Competent Cell is suitable for transgenic operations of cucurbitaceae, leguminosae, solanaceae and other plants.
Description: Intact Genomics BL21 chemically competent cells are suitable for transformation and routine protein expression from non-T7 vectors.Product Includes:BL21 competent cellspUC19 control DNARecovery medium
Description: Applications: C58C1 Chemically Competent Cell is suitable for transgenic operations of rosaceae, apocynaceae, leguminosae, solanaceae, astragalus, tobacco and other plants.
Description: Intact Genomics JM109 chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning.
Description: Intact Genomics JM109 chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning.
Description: Intact Genomics (ig®) HB101 chemically competent E. coli cells are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning. E. coli HB101 is a K12 x B hybrid strain, containing the recA13 mutation that minimizes recombination and helps insert stability. In addition, it carries the hsdS20(rB-mB-) restriction minus genotype which prevents cleavage of cloned DNA by endogenous restriction enzymes. HB101 strain does not support Alpha-complementation for blue/white screening
Description: Applications: GV3101 Chemically Competent Cell is suitable for transgenic operations of Arabidopsis, tobacco, corn, potatoes and other plants.
Description: Applications: MSU440 Chemically Competent Cell is suitable for transgenic operations of corn, tobacco, tea tree, artemisia annua and other plants.
Description: Intact Genomics (ig®) chemically competent MG1655 chemically competent cells are suitable for routine DNA transformation and other research purposes. MG1655 is the “wild type” K-12 strain with minimal genetic manipulation having only been cured of the temperate bacteriophage lambda and F plasmid by ultraviolet light and acridine orange
Description: Applications: Ar.A4 Electroporation Competent Cell is suitable for transgenic operations of corn, tobacco, carrot, licorice and other plants.
Description: BirA-transformed Competent E. coli cells are supplied as 10 x 50 µl vials._x000D_Strain BL21, a chemically competent E. coli B strain, contains an IPTG-inducible BirA_x000D_expression plasmid and constitutively-expressed streptomycin/spectinomycin resistance gene._x000D_These cells are compatible with most cloning vectors and are suitable for expression and_x000D_biotinylation of recombinant proteins using AviTag™ technology.
Description: Applications: Ar.Qual Electroporation Competent Cell is suitable for transgenic operations of corn, tobacco, tomato, citrus and other plants.
Description: Applications: K599 Electroporation Competent Cell is suitable for transgenic operations of cucurbitaceae, leguminosae, solanaceae and other plants.
Description: Applications: C58C1 Electroporation Competent Cell is suitable for transgenic operations of rosaceae, apocynaceae, leguminosae, solanaceae, astragalus, tobacco and other plants.
Description: Intact Genomics (ig®) Ar.A4 Chemically Competent Agrobacterium cells are made from a specific strain of Rhizobium rhizogenes (formerly Agrobacterium rhizogenes), Agrobacterium rhizogenes(kan R) Ar A4 Ri(agropine type). Agrobacterium rhizogenes is a soil-borne gram-negative bacterium that can infect most dicotyledons, a few monocotyledons and some gymnosperms. Ar.A4 Chemically Competent Agrobacterium are optimized for the highest transformation efficiencies and are useful for transgenic operations of corn, tobacco, carrot and other plants. Ar.A4 Agrobacterium rhizogenes strain contains agrobacterium-type Ri plasmid and displays kanamycin resistance.
Description: Intact Genomics (ig®) Ar.A4 Chemically Competent Agrobacterium cells are made from a specific strain of Rhizobium rhizogenes (formerly Agrobacterium rhizogenes), Agrobacterium rhizogenes(kan R) Ar A4 Ri(agropine type). Agrobacterium rhizogenes is a soil-borne gram-negative bacterium that can infect most dicotyledons, a few monocotyledons and some gymnosperms. Ar.A4 Chemically Competent Agrobacterium are optimized for the highest transformation efficiencies and are useful for transgenic operations of corn, tobacco, carrot and other plants. Ar.A4 Agrobacterium rhizogenes strain contains agrobacterium-type Ri plasmid and displays kanamycin resistance.
Description: Applications: GV3101 Electroporation Competent Cell is suitable for transgenic operations of Arabidopsis, tobacco, corn, potatoes and other plants.
Description: Applications: MSU440 Electroporation Competent Cell is suitable for transgenic operations of corn, tobacco, tea tree, artemisia annua and other plants.
Description: Intact Genomics chemically competent BL21(DE3) E. coli cells are suitable for transformation and routine protein expression.Product Includes:BL21 (DE3) competent cellspUC19 control DNARecovery medium
Description: Intact Genomics chemically competent BL21(DE3) E. coli cells are suitable for transformation and routine protein expression.Product Includes:BL21 (DE3) competent cellspUC19 control DNARecovery medium
Description: Intact Genomics chemically competent BL21(DE3) E. coli cells are suitable for transformation and routine protein expression.Product Includes:BL21 (DE3) competent cellspUC19 control DNARecovery medium
Description: Intact Genomics (ig®) Ar.Qual Chemically Competent Agrobacterium cells are made from a specific strain of Rhizobium rhizogenes (formerly Agrobacterium rhizogenes), Agrobacterium rhizogenes (str R,Cam R) Ar Qual Ri (agropine type). Agrobacterium rhizogenes is a soil-borne gram-negative bacterium that can infect most dicotyledons, a few monocotyledons and some gymnosperms. Ar.Qual Chemically Competent Agrobacterium are optimized for the highest transformation efficiencies and are useful for transgenic operations of corn, tobacco, tomato, citrus and other plants. Ar.Qual Agrobacterium rhizogenes strain contains agrobacterium-type Ri plasmid and displays streptomycin and chloramphenicol resistance.
Description: Intact Genomics (ig®) Ar.Qual Chemically Competent Agrobacterium cells are made from a specific strain of Rhizobium rhizogenes (formerly Agrobacterium rhizogenes), Agrobacterium rhizogenes (str R,Cam R) Ar Qual Ri (agropine type). Agrobacterium rhizogenes is a soil-borne gram-negative bacterium that can infect most dicotyledons, a few monocotyledons and some gymnosperms. Ar.Qual Chemically Competent Agrobacterium are optimized for the highest transformation efficiencies and are useful for transgenic operations of corn, tobacco, tomato, citrus and other plants. Ar.Qual Agrobacterium rhizogenes strain contains agrobacterium-type Ri plasmid and displays streptomycin and chloramphenicol resistance.
Description: The AGL1 strain (A. tumefaciens) has a C58 chromosomal background that carries an insertion mutation in its recA recombination gene which stabilizes recombinant plasmids. It also carries rifampicin and carbenicilin resistance in its genome for selection. AGL1 contains the Ti plasmid pTiBO542 from which the T-DNA region sequences have been deleted. Transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of Arabidopsis thaliana as well as maize and other monocots.Product Includes:• AGL1 Chemically Competent cells• pCAMBIA control DNA• Recovery medium
Description: The AGL1 strain (A. tumefaciens) has a C58 chromosomal background that carries an insertion mutation in its recA recombination gene which stabilizes recombinant plasmids. It also carries rifampicin and carbenicilin resistance in its genome for selection. AGL1 contains the Ti plasmid pTiBO542 from which the T-DNA region sequences have been deleted. Transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of Arabidopsis thaliana as well as maize and other monocots.Product Includes:• AGL1 Chemically Competent cells• pCAMBIA control DNA• Recovery medium
Description: C58C1 Chemically Competent Agrobacterium cells are optimized for the highest transformation efficiency and are useful for various applications. The chromosomal background of C58C1 is C58. C58 is cured of the Ti plasmid pTiC58 resulting in C58C1. C58C1 Competent cells may be useful for transgenic operations that involve Arabidopsis and other plants. This Agrobacterium strain is streptomycin and rifampicin resistant.Reagents Included:• C58C1 Chemically Competent Agrobacterium• DNA (pCAMBIA1391z, 500 pg/µl)• Recovery medium
Description: C58C1 Chemically Competent Agrobacterium cells are optimized for the highest transformation efficiency and are useful for various applications. The chromosomal background of C58C1 is C58. C58 is cured of the Ti plasmid pTiC58 resulting in C58C1. C58C1 Competent cells may be useful for transgenic operations that involve Arabidopsis and other plants. This Agrobacterium strain is streptomycin and rifampicin resistant.Reagents Included:• C58C1 Chemically Competent Agrobacterium• DNA (pCAMBIA1391z, 500 pg/µl)• Recovery medium
Description: Applications: ATCC15834 Electroporation Competent Cell is suitable for transgenic operations of gramineae, leguminous, tobacco and other plants.
Description: Intact Genomics JM109(DE3) chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. JM109(DE3) is useful for the high-level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter.
Description: Intact Genomics JM109(DE3) chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. JM109(DE3) is useful for the high-level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter.
Description: Applications: AGL1 (pSoup) Chemically Competent Cell is suitable for transgenic operations of Arabidopsis, tobacco, corn, potatoes and other plants.
Description: GV3101 strain (A. tumefaciens) has a C58 chromosomal background with rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) with gentamicin resistance. The GV3101 Ti plasmid has the T-DNA region sequences deleted and transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of several dicots such as Arabidopsis thaliana, tobacco, potato, and monocots like corn.Product Includes:• GV3101 Chemically cells• pCAMBIA control DNA• Recovery medium
Description: GV3101 strain (A. tumefaciens) has a C58 chromosomal background with rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) with gentamicin resistance. The GV3101 Ti plasmid has the T-DNA region sequences deleted and transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of several dicots such as Arabidopsis thaliana, tobacco, potato, and monocots like corn.Product Includes:• GV3101 Chemically cells• pCAMBIA control DNA• Recovery medium
Description: Intact Genomics EHA105 Chemically Competent Agrobacterium cells are optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. The EHA105 strain is useful for transgenic operations of rice, tobacco and other plants. EHA105 contains a rifampicin resistance gene (rif). EHA105 strain also contains an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, which contains the vir gene.Product Includes:• EHA105 Chemically Competent cells• pCAMBIA control DNA• Recovery medium
Description: Intact Genomics EHA105 Chemically Competent Agrobacterium cells are optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. The EHA105 strain is useful for transgenic operations of rice, tobacco and other plants. EHA105 contains a rifampicin resistance gene (rif). EHA105 strain also contains an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, which contains the vir gene.Product Includes:• EHA105 Chemically Competent cells• pCAMBIA control DNA• Recovery medium
Description: Intact Genomics LBA4404 Chemically Competent Agrobacterium cells are optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. The LBA4404 strain is useful for transgenic operations of tomatoes, tobacco and other plants. LBA4404 contains a rifampicin resistance gene (rif). LBA4404 strain also contains a octoprine-type Ti plasmid pAL4404 without self-transport function, which contains the vir gene.Product Includes:• LBA4404 Chemically Competent cells• pCAMBIA control DNA• Recovery medium
Description: Intact Genomics LBA4404 Chemically Competent Agrobacterium cells are optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. The LBA4404 strain is useful for transgenic operations of tomatoes, tobacco and other plants. LBA4404 contains a rifampicin resistance gene (rif). LBA4404 strain also contains a octoprine-type Ti plasmid pAL4404 without self-transport function, which contains the vir gene.Product Includes:• LBA4404 Chemically Competent cells• pCAMBIA control DNA• Recovery medium
Description: Applications: GV3101 (pSoup) Chemically Competent Cell is suitable for transgenic operations of Arabidopsis, tobacco, corn, potatoes and other plants.
Description: Intact Genomics (ig®) HB101 chemically competent E. coli cells are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning. E. coli HB101 is a K12 x B hybrid strain, containing the recA13 mutation that minimizes recombination and helps insert stability. In addition, it carries the hsdS20(rB-mB-) restriction minus genotype which prevents cleavage of cloned DNA by endogenous restriction enzymes. HB101 strain does not support Alpha-complementation for blue/white screening
Description: Intact Genomics ig™ 10B chemically competent cells (E. coli) are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. This derivative of DH10B provides the highest efficiency in the industry.Product Includes:ig™ 10B competent cellspUC19 control DNARecovery medium
Description: Intact Genomics ig™ 10B chemically competent cells (E. coli) are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. This derivative of DH10B provides the highest efficiency in the industry.Product Includes:ig™ 10B competent cellspUC19 control DNARecovery medium
Description: Applications: GV3101 (pSoup) Electroporation Competent Cell is suitable for transgenic operations of Arabidopsis, tobacco, corn, potatoes and other plants.
Description: Intact Genomics Chemically Competent Agrobacterium Combo Pack is perfect for scientists who require multiple agrobacterium competent cell strains for their research. The ElectroCompetent Agrobacterium Combo Pack contains 3 50µl aliquots of GV3101, AGL1, EHA105 and LBA4404 along with pCambia and our proprietary Agro Recovery Media.The GV3101 strain has a C58 chromosomal background with rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) with gentamicin resistance. The GV3101 Ti plasmid has the T-DNA region sequences deleted and transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of several dicots such as Arabidopsis thaliana, tobacco, potato, and monocots like corn.The AGL1 strain has a C58 chromosomal background that carries an insertion mutation in its recA recombination gene which stabilizes recombinant plasmids. It also carries rifampicin and carbenicilin resistance in its genome for selection. AGL1 contains the Ti plasmid pTiBO542 from which the T-DNA region sequences have been deleted. Transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of Arabidopsis thaliana as well as maize and other monocots.The LBA4404 strain is useful for transgenic operations of tomatoes, tobacco and other plants. LBA4404 contains a rifampicin resistance gene (rif). LBA4404 strain also contains a octoprine-type Ti plasmid pAL4404 without self-transport function, which contains the vir gene.The EHA105 strain is useful for transgenic operations of rice, tobacco and other plants. EHA105 contains a rifampicin resistance gene (rif). EHA105 strain also contains an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, which contains the vir gene.
Description: Intact Genomics (ig®) chemically competent BL21(DE3)pLysS chemically competent cells are suitable for transformation and routine protein expression. The pLysS plasmid produces T7 lysozyme that reduces base level expression of the gene of interest, subsequently allowing for tighter control of expression and is therefore suitable for expression of toxic genes. BL21(DE3)pLysS chemically competent cells also carry the chloramphenicol resistance gene.
Description: Intact Genomics (ig®) chemically competent BL21(DE3)pLysS chemically competent cells are suitable for transformation and routine protein expression. The pLysS plasmid produces T7 lysozyme that reduces base level expression of the gene of interest, subsequently allowing for tighter control of expression and is therefore suitable for expression of toxic genes. BL21(DE3)pLysS chemically competent cells also carry the chloramphenicol resistance gene.
Description: Intact Genomics chemically competent DL39 (DE3) E. coli cells are specific for transformation and protein expression in order to uniformly and specifically label :e.g. phenylalanine or leucine residues. DL39 (DE3) can also be used to reduce NMR cross-labeling via transaminase activity for valine, leucine, isoleucine, aspartate, phenylalanine, tyrosine and tryptophan residuesProduct Includes:DL39 (DE3) competent cellspUC19 control DNARecovery medium
Description: Intact Genomics chemically competent BL21(DE3) E. coli cells are suitable for transformation and routine protein expression.Product Includes:BL21 (DE3) competent cellspUC19 control DNARecovery medium
Description: Applications: GV3101 (pSoup-p19) Chemically Competent Cell is suitable for transgenic operations of Arabidopsis, tobacco, corn, potatoes and other plants.
Description: Intact Genomics 5-alpha chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. ig™ 5-alpha chemically competent cells are at least twice the transformation efficiency of the nearest competitor.Product Includes:ig™ 5-alpha competent cellspUC19 control DNARecovery medium
Description: Intact Genomics 5-alpha chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. ig™ 5-alpha chemically competent cells are at least twice the transformation efficiency of the nearest competitor.Product Includes:ig™ 5-alpha competent cellspUC19 control DNARecovery medium
Description: Intact Genomics 5-alpha chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. ig™ 5-alpha chemically competent cells are at least twice the transformation efficiency of the nearest competitor.Product Includes:ig™ 5-alpha competent cellspUC19 control DNARecovery medium
Description: Intact Genomics 5-alpha chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. ig™ 5-alpha chemically competent cells are at least twice the transformation efficiency of the nearest competitor.Product Includes:ig™ 5-alpha competent cellspUC19 control DNARecovery medium
Description: Intact Genomics 5-alpha chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. ig™ 5-alpha chemically competent cells are at least twice the transformation efficiency of the nearest competitor.Product Includes:ig™ 5-alpha competent cellspUC19 control DNARecovery medium
Description: Intact Genomics 5-alpha chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. ig™ 5-alpha chemically competent cells are at least twice the transformation efficiency of the nearest competitor.Product Includes:ig™ 5-alpha competent cellspUC19 control DNARecovery medium
Description: Intact Genomics 5-alpha chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. ig™ 5-alpha chemically competent cells are at least twice the transformation efficiency of the nearest competitor.Product Includes:ig™ 5-alpha competent cellspUC19 control DNARecovery medium
Description: Intact Genomics (ig®) MSU440 Chemically Competent Agrobacterium cells are made from a specific strain of Rhizobium rhizogenes (formerly Agrobacterium rhizogenes), Agrobacterium rhizogenes(str R) MSU440 Ri (agropine type). Agrobacterium rhizogenes is a soil-borne gram-negative bacterium that can infect most dicotyledons, a few monocotyledons and some gymnosperms. MSU440 Chemically Competent Agrobacterium are optimized for the highest transformation efficiencies and are useful for transgenic operations of corn, tobacco, wormwood, tea tree and other plants. MSU440 Agrobacterium rhizogenes strain contains agrobacterium-type Ri plasmid and displays streptomycin resistance.
Description: Intact Genomics (ig®) DirectPlate™ Competent cells offer simple, fast, and robust results for your DNA transformation needs. DirectPlate™ 10B chemically competent E. coli cells are a perfect choice for researchers looking to simplify their transformation workflow by eliminating heat shock, lengthy incubations, and time-consuming outgrowth procedures. Simply mix and directly plate! DirectPlate™ 10B chemically competent E. coli cells provide higher transformation efficiency than any competitor‘s similar product and are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning.Product Includes:DirectPlate™ 10B Chemically Competent CellspUC19 control DNA
Description: Intact Genomics (ig®) DirectPlate™ Competent cells offer simple, fast, and robust results for your DNA transformation needs. DirectPlate™ 10B chemically competent E. coli cells are a perfect choice for researchers looking to simplify their transformation workflow by eliminating heat shock, lengthy incubations, and time-consuming outgrowth procedures. Simply mix and directly plate! DirectPlate™ 10B chemically competent E. coli cells provide higher transformation efficiency than any competitor‘s similar product and are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning.Product Includes:DirectPlate™ 10B Chemically Competent CellspUC19 control DNA