Klonen van een HcCreb-gen en analyse van de effecten ervan op paarlemoerkleur en melaninesynthese in Hyriopsis cumingii
Creb (Cyclic AMP response ingredient bindend eiwit) is een nucleaire regulerende issue die transcriptie reguleert door autofosforylering. In melanocyten binden de overeenkomstige elementen van cAMP aan het Creb-eiwit voor autofosforylering en activeren ze MITF (Microphthalmia-associated transcription issue) . MITF stimuleert Tyrosine (tyr) om melanocyten te induceren om te differentiëren in eumelanine en pheomelanine. In deze studie werd een HcCreb-gen in Hyriopsis cumingii gekloond en de effecten ervan op de melaninesynthese en de paarlemoerkleur werden bestudeerd. HcCreb kwam tot expressie in zowel paarse als witte mosselen en er was een important verschil in expressie tussen adductoren (p<0,01) en mantelweefsel (p<0,05) .
Andere weefsels vertoonden geen significante verschillen (behalve voor kieuwweefsel), en in het algemeen was het niveau van mRNA-expressie hoger in paarse mosselen dan in witte mosselen. In zowel witte als paarse mosselen was de expressie in kieuwweefsel het hoogst, gevolgd door de mantel. Er werden sterke en specifieke mRNA-signalen gedetecteerd in de dorsale epitheelcellen van de palliale laag van de mantel, wat aangeeft dat HcCreb mogelijk betrokken is bij de vorming van parelmoer. Na behandeling met arbutine nam de expressie van HcCreb important af. Door de veranderingen in het melaninegehalte van de mantel verder te testen, werd gevonden dat het melaninegehalte na behandeling met arbutine important afnam in vergelijking met de controlegroep (p<0,05) . Er wordt gespeculeerd dat het HcCreb-gen een rol speelt in het proces van melaninesynthese en parelkleurvorming in H. cumingii.
Een Ab Initio meervoudige kloneringsmethode voor niet-adiabatische moleculaire dynamiek in opgewonden toestand in NWChem
De latest ontwikkelde ab initio a number of cloning (AIMC) -benadering op foundation van de multiconfigurationele Ehrenfest (MCE) -methode biedt een krachtige en nauwkeurige manier om de dynamiek van de aangeslagen toestand van moleculaire systemen te beschrijven. De AIMC-methode is een gecontroleerde benadering van niet-adiabatische dynamiek met een bijzondere kracht in de juiste beschrijving van decoherentie-effecten vanwege de vertakking van trillingsgolfpakketten bij een overweg. Hier rapporteren we een nieuwe implementatie van het AIMC-algoritme in het open supply NWChem computationele chemieprogramma.
Het raamwerk combineert lineaire-responstijdafhankelijke dichtheidsfunctionaaltheorie met Ehrenfest-gemiddelde-veldtheorie om de bewegingsvergelijkingen voor klassieke trajecten te bepalen . De multidimensionale golffunctie wordt ontleed in een superpositie van Gaussiaanse coherente toestanden geleid door Ehrenfest-trajecten (dwz MCE-benadering), die kunnen klonen met volledig kwantummechanische amplituden en fasen. Door gebruik te maken van een efficiënte op tijd gebaseerde niet-adiabatische koppelingsbenadering binnen de AIMC-methode, worden alle waarneembare waarden on-the-fly berekend in het niet-adiabatische moleculaire dynamicaproces.
Als representatief voorbeeld passen we onze implementatie toe om de ultrasnelle foto-geïnduceerde elektronische en vibrerende energieoverdracht in een pyridinemolecuul te bestuderen. De effecten van de kloonprocedure op elektronische en vibrationele coherentie, relaxatie en unidirectionele energieoverdracht worden besproken. Deze nieuwe AIMC-implementatie biedt een niet-adiabatisch moleculaire dynamica-raamwerk op hoog niveau voor het simuleren van foto-geëxciteerde dynamica in complexe moleculaire systemen en experimenteel relevante ultrasnelle spectroscopische sondes, zoals niet-lineaire coherente optische en röntgensignalen.
osteoporoseliga
Cold Fusion Cloning Kit with Competent Cells (50 rxns) International Sales Only
Description: The Quick PCR™ Plus Assembly Kit is used as a molecular cloning tool to assemble long DNA fragment from multiple smaller fragments, or to insert DNA into a plasmid in a single reaction. The main kit component is a ready-to-use mix of enzymes in reaction buffer at a 2-fold concentration. This kit also includes chemically competent E. coli cells (another version of the kit does not include competent cells, BPS Bioscience #78531).
Description: The Quick PCR™ Plus Assembly Kit is used as a molecular cloning tool to assemble long DNA fragment from multiple smaller fragments, or to insert DNA into a plasmid in a single reaction. The main kit component is a ready-to-use mix of enzymes in reaction buffer at a 2-fold concentration. This kit also includes chemically competent E. coli cells (another version of the kit does not include competent cells, BPS Bioscience #78531).
Description: BirA-transformed Competent E. coli cells are supplied as 10 x 50 µl vials._x000D_Strain BL21, a chemically competent E. coli B strain, contains an IPTG-inducible BirA_x000D_expression plasmid and constitutively-expressed streptomycin/spectinomycin resistance gene._x000D_These cells are compatible with most cloning vectors and are suitable for expression and_x000D_biotinylation of recombinant proteins using AviTag™ technology.
Description: A sandwich quantitative ELISA assay kit for detection of Human Fusion (FUS) in samples from tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Fusion (FUS) in samples from tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Fusion (FUS) in samples from tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Fusion (FUS) in samples from tissue homogenates or other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Fusion (FUS) in tissue homogenates, cell lysates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Fusion (FUS) in tissue homogenates, cell lysates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Fusion (FUS) in tissue homogenates, cell lysates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Fusion (FUS) in tissue homogenates, cell lysates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Fusion (FUS) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Fusion (FUS) in Tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Fusion (FUS) in Tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Fusion (FUS) in Tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Fusion (FUS) in Tissue homogenates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Fusion (FUS) in samples from Tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.
Description: Human secreted CD28-Fc fusion protein, also known as TP44, GenBank Accession No. NM_006139, a.a 19-152, expressed in a HEK293 cell expression system. MW = 41.4 kDa (monomer). This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
Description: Human herpesvirus entry mediator A, also known as HVEM, TNFRSF14, CD270, and HVEA, GenBank Accession No. NM_003820, a.a. 37-202 fused to human IgG1 Fc, expressed in a HEK293 cell expression system. MW = 44.2 kDa. This protein runs at a higher M.W. by SDSPAGE due to glycosylation.
Description: Human secreted CD40, Fc fusion protein, also known as Tumor Necrosis Factor Receptor Superfamily Member 5, TNFRSF5, and Bp50, GenBank Accession No. NM_001250, a.a. 21-193 expressed in a HEK293 cell expression system. MW = 45.9 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human secreted CD27, Fc fusion protein, also known as Tumor Necrosis Factor Receptor Superfamily Member 7, TNFRSF7, and T14, GenBank Accession No. NM_001242, a.a. 21-192 expressed in a HEK293 cell expression system. MW = 45.8 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human CD47, Fc fusion protein, also known as leukocyte surface antigen CD47, Rh-related antigen, integrin-associated protein, antigenic surface determinant protein OA3, antigen identified by monoclonal antibody 1D8, IAP, and MER6. GenBank Accession No. NM_001777, a.a. 19-139 expressed in a HEK293 cell expression system. MW = 40 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human CD44, also known as epican, extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, or phagocytic glyco-protein I (PGP-1), GenBank Accession No. NM_000610, a.a. 21-220, fused with Fc region of human IgG, expressed in a HEK293 cell expression system. MW = 48.7 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Eppendorf Multiporator Helix Fusion Chamber For Cell Fusion
Description: A sandwich ELISA kit for detection of Fusion from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: A sandwich ELISA kit for detection of Fusion from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: Human T-cell immunoreceptor with Ig and_x000D_ITIM domains (TIGIT), also known as V-set_x000D_and immunoglobulin domain-containing_x000D_protein 9, VSIG9, V-set and transmembrane_x000D_domain-containing protein 3, and VSTM3,_x000D_GenBank Accession No. NM_173799, a.a._x000D_22-141 fused to Fc region of human IgG,_x000D_expressed in a HEK293 cell expression_x000D_system. MW = 39.7 kDa. This protein runs_x000D_at a higher MW by SDS-PAGE due to_x000D_glycosylation.
Description: Human secreted TIM-3, Fc fusion protein, also known as T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, T-cell and immunoglobulin and mucin domain-containing protein 3, TIMD-3, Hepatitis A virus cellular receptor 2, and HAVCR-2. GenBank Accession No. NM_032782.4, a.a. 22-200 expressed in a HEK293 cell expression system. MW = 46.5 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human secreted lymphotoxin beta receptor_x000D_(LTBR)-Fc fusion protein, also known as_x000D_Tumor Necrosis Factor C Receptor_x000D_(TNFCR), and CD18, GenBank Accession_x000D_No. NM_002342, a.a 28-219, expressed in_x000D_a HEK293 cell expression system. MW = 48_x000D_kDa (monomer). This protein runs at a_x000D_higher apparent M.W. by SDS-PAGE due to_x000D_glycosylation.
Description: Mouse secreted lymphotoxin beta receptor_x000D_(mLTBR)-Fc fusion protein, also known as,_x000D_Tumor Necrosis Factor C Receptor_x000D_(TNFCR), and CD18, GenBank Accession_x000D_No. NM_010736, a.a 28-221, expressed in_x000D_a HEK293 cell expression system. MW =_x000D_48.8 kDa (monomer). Endotoxin level_x000D_<0.001 EU/ug.This protein runs at a higher_x000D_apparent M.W. by SDS-PAGE due to_x000D_glycosylation.
Description: Human B- and T-lymphocyte attenuator (BTLA)-Fc fusion protein, also known as CD272, GenBank Accession No. NM_181780, a.a. 31-150, expressed in a HEK293 cell expression system. MW = 40.3 kDa (monomer). This protein runs at a higher apparent M.W. by SDS-PAGE due to glycosylation.
Description: Human secreted LAG3, Fc fusion protein, also known as Lymphocyte-Activation Gene 3 and CD223. GenBank Accession No. NM_002286, a.a. 23-450 expressed in a HEK293 cell expression system. MW = 73.1 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human secreted GITR, Fc fusion protein, also known as Glucocorticoid-induced TNFR-Related Protein, Tumor Necrosis Factor Receptor Superfamily member 18, TNFRSF18, Activation-Inducible TNFR Family Receptor, AITR, and CD357, GenBank Accession No. NM_004195, a.a. 26-161 expressed in a HEK293 cell expression system. MW = 41.2 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human secreted OX40, Fc fusion protein,_x000D_also known as Tumor Necrosis Factor_x000D_Receptor Superfamily Member 4,_x000D_TNFRSF4, and CD134, GenBank_x000D_Accession No. NM_003327, a.a. 29-216_x000D_expressed in a HEK293 cell expression_x000D_system. MW = 46.8 kDa (monomer). This_x000D_protein runs at a higher MW by SDS-PAGE_x000D_due to glycosylation.
Description: Human ICOS, Fc fusion protein, also known as inducible T-cell costimulator and CD antigen 278, activation-inducible lymphocyte immunomediatory molecule, AILIM, CD278, and CVID1. GenBank Accession No. NM_012092, a.a. 21-140 expressed in a HEK293 cell expression system. MW = 40.2 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Rhesus monkey angiotensin I converting enzyme 2 (ACE2), also known as ACEH, Genbank Accession No.: ACI04553.1, a.a. 18-739, fused at the C-terminus of the Fc portion of human IgG1, expressed in a HEK293 expression system, MW= 119 kDa. This protein runs at a higher MW due to glycosylation.
Description: Rhesus monkey angiotensin I converting enzyme 2 (ACE2), also known as ACEH, Genbank Accession No.: ACI04553.1, a.a. 18-739, fused at the C-terminus of the Fc portion of human IgG1, expressed in a HEK293 expression system, MW= 119 kDa. This protein runs at a higher MW due to glycosylation.
Description: Human secreted CTLA4 , Fc fusion protein, also known as Cytotoxic T-lymphocyte-associated protein 4 and CD152. GenBank Accession No. NM_005214, a.a. 36-162 expressed in a HEK293 cell expression system. MW = 40.3 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human CD226 antigen also_x000D_known as DNAX accessory molecule 1_x000D_(DNAM-1), GenBank Accession No._x000D_NM_006566, a.a. 19-247 fused to Fc region_x000D_of human IgG1, expressed in a HEK293 cell_x000D_expression system. MW = 52.8 kDa_x000D_(monomer). This protein runs at a higher_x000D_MW by SDS-PAGE due to glycosylation.
Description: Secreted frizzled receptor (FZD1)-Fc fusion_x000D_protein and WNT receptor, also known as_x000D_FZD1, GenBank Accession No.BC_051271,_x000D_a.a. 73-253, expressed in a HEK293 cell_x000D_expression system. MW = 46.4 kDa_x000D_(monomer). This protein runs at a higher_x000D_apparent M.W. by SDS-PAGE due to_x000D_glycosylation.
Description: Secreted frizzled receptor (FZD4)-Fc fusion_x000D_protein and WNT receptor, also known as_x000D_FZD4, GenBank Accession No._x000D_BC_114527, a.a. 37-180, expressed in a_x000D_HEK293 cell expression system. MW = 42.9_x000D_kDa (monomer). This protein runs at a_x000D_higher apparent M.W. by SDS-PAGE due to_x000D_glycosylation.
Description: Secreted frizzled receptor (FZD7)-Fc fusion_x000D_protein and WNT receptor, also known as_x000D_FZD7, GenBank Accession No._x000D_BC_015915, a.a. 33-185, expressed in a_x000D_HEK293 cell expression system. MW = 43.3_x000D_kDa (monomer). This protein runs at a_x000D_higher apparent M.W. by SDS-PAGE due to_x000D_glycosylation.
Description: Secreted frizzled receptor (FZD10)-Fc_x000D_fusion protein and WNT receptor, also_x000D_known as FZD10, GenBank Accession No._x000D_BC_074997, a.a. 21-161, expressed in a_x000D_HEK293 cell expression system. MW = 42.7_x000D_kDa (monomer). This protein runs at a_x000D_higher apparent M.W. by SDS-PAGE due to_x000D_glycosylation.
Description: Human secreted B7-1-Fc fusion protein, also known as CD80. GenBank Accession No. NM_005191, a.a. 35-242 expressed in a HEK293 cell expression system. MW = 50.5 kDa (monomer). This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
Description: Human secreted B7-2, Fc fusion protein, also known as T-lymphocyte activation antigen CD86, B7.2, FUN-1, B70, BU63, and CD86. GenBank Accession No. NM_006889, a.a. 20-239 expressed in a HEK293 cell expression system. MW = 51.9 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human secreted B7-H5, Fc fusion protein, also known as Gi24, Dies1, VISTA, and SISP1, GenBank Accession No. NM_022153, a.a. 33-194 expressed in a HEK293 cell expression system. MW = 46.7 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human MICA, also known as, MHC Class I Polypeptide-Related Sequence A, PERB11.1, and Stress Inducible Class I Homolog, GenBank Accession No. NM_001177519, a.a. 23-308, fused at the C-terminus to the Fc portion of human IgG1 followed by a C-terminal Avi-Tag™ and expressed in a HEK293 cell expression system. MW=62 kDa.
Description: Human B- and T-lymphocyte attenuator (BTLA), also known as CD272, with C-terminal Avi-TagTM fused to the Fc-region of Human IgG1. GenBank Accession No. NM_181780, a.a. 31-150, expressed in a HEK293 cell expression system. MW = 42 kDa (monomer). This protein runs at a higher apparent M.W. by SDS-PAGE due to glycosylation.
Description: Human secreted CD33, also known as Sialic Acid Binding Ig Like Lectin 3, Gp67 and Myeloid Cell Surface Antigen CD33, GenBank Accession No. NM_001772, a.a. 1-259, fused at the C-terminus of the Fc portion of human IgG1, with C-terminal Avi-tag™ , expressed in a HEK293 cell expression system. MW = 57 kDa.
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Glucosiden in biodiesel
Biodiesels op foundation van plantaardige olie hebben een groot kwaliteitsprobleem vanwege de aanwezigheid van precipitaten gevormd door sterylglucosiden, die filters en injectoren van dieselmotoren verstoppen . Er is een efficiënte, schaalbare en kosteneffectieve methode ontwikkeld om sterylglucosiden te hydrolyseren met behulp van thermostabiele enzymen. Hier worden methoden gepresenteerd om enzymen met SGase- activiteit te ontdekken, tot expressie te brengen in recombinante micro-organismen en te produceren , evenals methoden om biodiesel met dergelijke enzymen te behandelen en om het gehalte aan sterylglucosiden in biodieselmonsters te meten.
Klonering , karakterisering en expressie van een gen dat codeert voor endo-1,4-β-xylanase van de schimmel Termitomyces clypeatus
Enzymatische afbraak van hemi-cellulosesubstraten heeft de laatste tijd veel industriële aandacht gekregen. Volledige enzymatische afbraak van complexe en weerbarstige hemicellulose vereist een enzymatische cocktail die voornamelijk bestaat uit endo-1,4-β-xylanase (xyl), β-xylosidase, arabinofuranosidase enz . Dit artikel rapporteert voor het eerst de identificatie, klonering, expressie en gedeeltelijke karakterisering van een krachtig endo-1,4- ,-xylanasegen (pxyl) van de paddenstoel Termitomyces clypeatus (TC) in E. coli en S. cerevisiae .
Het cDNA voor pxyl bleek 678 bp te zijn, wat op zijn beurt aanleiding geeft tot een voorlopereiwit (Pxyl) van 225 aminozuren lang wanneer het wordt gekloneerd in een prokaryotische expressievector. Om bovendien te karakteriseren, werd het cDNA ook tot expressie gebracht in S. cerevisiae. Bioinformatica-onderzoek voorspelde dat de Pxyl een leiderpeptide van 19 aminozuren bevat dat post-translationele modificaties mogelijk maakt, waaronder glycosylering, evenals de efficiënte secretie ervan in het medium. Van het recombinante eiwit is gevonden dat het een lid is van de GH11-familie met twee verre glutaminezuren als katalytische residuen. Dit rapport beschrijft nog een nieuwe en krachtige bron van xylanase voor commerciële exploitatie door de industrie in de toekomst.
Klonen , sequentieanalyse en weefselexpressie van paraoxonase van zijdeaapjes 1
Paraoxonase (PON) speelt een rol in het metabolisme van organofosfaatxenobiotica en geneesmiddelen. Ondanks het belang van zijdeaapjes voor onderzoek naar het metabolisme en de farmacokinetiek van geneesmiddelen, is paraoxonase van zijdeaapjes nog niet volledig gekarakteriseerd. Bijgevolg identificeerden we het PON1-gen in het genoom van de zijdeaapje door middel van sequentiehomologie-analyse. Marmoset PON1 cDNA met een open leesraam (1065 bp) werd met succes gekloneerd uit de lever van marmoset door middel van een reverse transcriptie-polymerase kettingreactie. De afgeleide aminozuursequentie (355 aminozuren) heeft ongeveer 93% identiteit met de menselijke ortholoog en bevat belangrijke aminozuurresiduen voor substraatbinding en calciumioncoördinatie.
Volgens een fylogenetische increase van PON1-aminozuursequenties, geconstrueerd met behulp van gegevens van zeven diersoorten, ligt zijdeaapje PON1 dichter bij menselijk PON1 dan bij de PON1-orthologen van proefdieren zoals varkens, konijnen, ratten en muizen. Marmoset PON1-mRNA werd voornamelijk tot expressie gebracht in de lever van de vijf onderzochte weefsels. Marmoset PON1-eiwit uitgescheiden in plasma werd gedetecteerd door immunoblotting. De paraoxon-hydrolyserende activiteit in plasma was hoger bij zijdeaapjes dan bij mensen . Op foundation van deze gegevens hebben we geconcludeerd dat zijdeaapje en humaan PON1 vergelijkbare kenmerken hebben met betrekking tot de genomische structuur, aminozuursequenties en weefseldistributie.
Een nieuwe pH en thermotolerante halofiele alfa-amylase van matige halofiele Nesterenkonia sp. stam F: genanalyse, moleculaire klonering , heterologe expressie en biochemische karakterisering
Een nieuwe pH en thermotolerante halofiele alfa-amylase van een matig halofiele bacterie, Nesterenkonia sp. stam F, werd gekloneerd en tot expressie gebracht in Escherichia coli . 16S rRNA-sequentie van de stam deelde 99,46% overeenkomsten met nauw verwante typesoorten. Ook deelde de genoomsequentie ANI-waarden van minder dan 92% en dDDH-waarden van minder dan 52% met de nauw verwante typesoorten. Bijgevolg wordt voorgesteld dat stam F een nieuwe soort vertegenwoordigt. Het AmyF-gen was 1390 bp lang en codeert voor een alfa-amylase van 463 aminozuurresiduen met een pI van 4,62. De afgeleide AmyF deelde een zeer lage sequentieovereenkomst (< 24%) met functioneel gekarakteriseerde recombinante halofiele alfa-amylasen.
Het recombinante alfa-amylase werd met succes gezuiverd uit Ni-NTA-kolommen met een molecuulmassa van ongeveer 52 KDa op natriumdodecylsulfaat-polyacrylamidegelelektroforese. Het enzym was actief over een breed temperatuurbereik (25-75°C) en pH (4-9) met een optimale activiteit bij respectievelijk 45°C en 7,5. Ook werd, hoewel het actief was bij verschillende concentraties NaCl en KCl (0-Four M), een toenemende activiteit van het enzym waargenomen bij toenemende concentratie van deze zouten. Lage concentraties Ca2 + -ionen hadden geen activerend impact, maar hoge concentraties van het ion (40-200 mM) versterkten de activiteit van AmyF.
De enzymactiviteit werd verhoogd door toenemende concentraties van Mg2 + , Zn2 + , Hg2 + en Fe3 + . Het werd echter alleen geremd bij zeer hoge concentraties van deze metaalionen. Cu2 + verminderde de amylase-activiteit niet en de hoogste activiteit werd waargenomen bij 100 mM van het ion. Deze eigenschappen duiden op brede potentiële toepassingen van dit recombinante enzym in zetmeelverwerkende industrieën. Dit is de eerste isolatie , klonering en karakterisering van een gen dat codeert voor alfa-amylase van het geslacht Nesternkonia.
Cold Fusion Cloning Kit with Competent Cells (50 rxns) International Sales Only
Description: The Quick PCR™ Plus Assembly Kit is used as a molecular cloning tool to assemble long DNA fragment from multiple smaller fragments, or to insert DNA into a plasmid in a single reaction. The main kit component is a ready-to-use mix of enzymes in reaction buffer at a 2-fold concentration. This kit also includes chemically competent E. coli cells (another version of the kit does not include competent cells, BPS Bioscience #78531).
Description: The Quick PCR™ Plus Assembly Kit is used as a molecular cloning tool to assemble long DNA fragment from multiple smaller fragments, or to insert DNA into a plasmid in a single reaction. The main kit component is a ready-to-use mix of enzymes in reaction buffer at a 2-fold concentration. This kit also includes chemically competent E. coli cells (another version of the kit does not include competent cells, BPS Bioscience #78531).
Description: BirA-transformed Competent E. coli cells are supplied as 10 x 50 µl vials._x000D_Strain BL21, a chemically competent E. coli B strain, contains an IPTG-inducible BirA_x000D_expression plasmid and constitutively-expressed streptomycin/spectinomycin resistance gene._x000D_These cells are compatible with most cloning vectors and are suitable for expression and_x000D_biotinylation of recombinant proteins using AviTag™ technology.
Description: A sandwich quantitative ELISA assay kit for detection of Human Fusion (FUS) in samples from tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Fusion (FUS) in samples from tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Fusion (FUS) in samples from tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Fusion (FUS) in samples from tissue homogenates or other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Fusion (FUS) in tissue homogenates, cell lysates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Fusion (FUS) in tissue homogenates, cell lysates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Fusion (FUS) in tissue homogenates, cell lysates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Fusion (FUS) in tissue homogenates, cell lysates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Fusion (FUS) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Fusion (FUS) in Tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Fusion (FUS) in Tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Fusion (FUS) in Tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Fusion (FUS) in Tissue homogenates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Fusion (FUS) in samples from Tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.
Description: Human secreted CD28-Fc fusion protein, also known as TP44, GenBank Accession No. NM_006139, a.a 19-152, expressed in a HEK293 cell expression system. MW = 41.4 kDa (monomer). This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
Description: Human herpesvirus entry mediator A, also known as HVEM, TNFRSF14, CD270, and HVEA, GenBank Accession No. NM_003820, a.a. 37-202 fused to human IgG1 Fc, expressed in a HEK293 cell expression system. MW = 44.2 kDa. This protein runs at a higher M.W. by SDSPAGE due to glycosylation.
Description: Human secreted CD40, Fc fusion protein, also known as Tumor Necrosis Factor Receptor Superfamily Member 5, TNFRSF5, and Bp50, GenBank Accession No. NM_001250, a.a. 21-193 expressed in a HEK293 cell expression system. MW = 45.9 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human secreted CD27, Fc fusion protein, also known as Tumor Necrosis Factor Receptor Superfamily Member 7, TNFRSF7, and T14, GenBank Accession No. NM_001242, a.a. 21-192 expressed in a HEK293 cell expression system. MW = 45.8 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human CD47, Fc fusion protein, also known as leukocyte surface antigen CD47, Rh-related antigen, integrin-associated protein, antigenic surface determinant protein OA3, antigen identified by monoclonal antibody 1D8, IAP, and MER6. GenBank Accession No. NM_001777, a.a. 19-139 expressed in a HEK293 cell expression system. MW = 40 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human CD44, also known as epican, extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, or phagocytic glyco-protein I (PGP-1), GenBank Accession No. NM_000610, a.a. 21-220, fused with Fc region of human IgG, expressed in a HEK293 cell expression system. MW = 48.7 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Eppendorf Multiporator Helix Fusion Chamber For Cell Fusion
Description: A sandwich ELISA kit for detection of Fusion from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: A sandwich ELISA kit for detection of Fusion from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: Human T-cell immunoreceptor with Ig and_x000D_ITIM domains (TIGIT), also known as V-set_x000D_and immunoglobulin domain-containing_x000D_protein 9, VSIG9, V-set and transmembrane_x000D_domain-containing protein 3, and VSTM3,_x000D_GenBank Accession No. NM_173799, a.a._x000D_22-141 fused to Fc region of human IgG,_x000D_expressed in a HEK293 cell expression_x000D_system. MW = 39.7 kDa. This protein runs_x000D_at a higher MW by SDS-PAGE due to_x000D_glycosylation.
Description: Human secreted TIM-3, Fc fusion protein, also known as T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, T-cell and immunoglobulin and mucin domain-containing protein 3, TIMD-3, Hepatitis A virus cellular receptor 2, and HAVCR-2. GenBank Accession No. NM_032782.4, a.a. 22-200 expressed in a HEK293 cell expression system. MW = 46.5 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human secreted lymphotoxin beta receptor_x000D_(LTBR)-Fc fusion protein, also known as_x000D_Tumor Necrosis Factor C Receptor_x000D_(TNFCR), and CD18, GenBank Accession_x000D_No. NM_002342, a.a 28-219, expressed in_x000D_a HEK293 cell expression system. MW = 48_x000D_kDa (monomer). This protein runs at a_x000D_higher apparent M.W. by SDS-PAGE due to_x000D_glycosylation.
Description: Mouse secreted lymphotoxin beta receptor_x000D_(mLTBR)-Fc fusion protein, also known as,_x000D_Tumor Necrosis Factor C Receptor_x000D_(TNFCR), and CD18, GenBank Accession_x000D_No. NM_010736, a.a 28-221, expressed in_x000D_a HEK293 cell expression system. MW =_x000D_48.8 kDa (monomer). Endotoxin level_x000D_<0.001 EU/ug.This protein runs at a higher_x000D_apparent M.W. by SDS-PAGE due to_x000D_glycosylation.
Description: Human B- and T-lymphocyte attenuator (BTLA)-Fc fusion protein, also known as CD272, GenBank Accession No. NM_181780, a.a. 31-150, expressed in a HEK293 cell expression system. MW = 40.3 kDa (monomer). This protein runs at a higher apparent M.W. by SDS-PAGE due to glycosylation.
Description: Human secreted LAG3, Fc fusion protein, also known as Lymphocyte-Activation Gene 3 and CD223. GenBank Accession No. NM_002286, a.a. 23-450 expressed in a HEK293 cell expression system. MW = 73.1 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human secreted GITR, Fc fusion protein, also known as Glucocorticoid-induced TNFR-Related Protein, Tumor Necrosis Factor Receptor Superfamily member 18, TNFRSF18, Activation-Inducible TNFR Family Receptor, AITR, and CD357, GenBank Accession No. NM_004195, a.a. 26-161 expressed in a HEK293 cell expression system. MW = 41.2 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human secreted OX40, Fc fusion protein,_x000D_also known as Tumor Necrosis Factor_x000D_Receptor Superfamily Member 4,_x000D_TNFRSF4, and CD134, GenBank_x000D_Accession No. NM_003327, a.a. 29-216_x000D_expressed in a HEK293 cell expression_x000D_system. MW = 46.8 kDa (monomer). This_x000D_protein runs at a higher MW by SDS-PAGE_x000D_due to glycosylation.
Description: Human ICOS, Fc fusion protein, also known as inducible T-cell costimulator and CD antigen 278, activation-inducible lymphocyte immunomediatory molecule, AILIM, CD278, and CVID1. GenBank Accession No. NM_012092, a.a. 21-140 expressed in a HEK293 cell expression system. MW = 40.2 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Rhesus monkey angiotensin I converting enzyme 2 (ACE2), also known as ACEH, Genbank Accession No.: ACI04553.1, a.a. 18-739, fused at the C-terminus of the Fc portion of human IgG1, expressed in a HEK293 expression system, MW= 119 kDa. This protein runs at a higher MW due to glycosylation.
Description: Rhesus monkey angiotensin I converting enzyme 2 (ACE2), also known as ACEH, Genbank Accession No.: ACI04553.1, a.a. 18-739, fused at the C-terminus of the Fc portion of human IgG1, expressed in a HEK293 expression system, MW= 119 kDa. This protein runs at a higher MW due to glycosylation.
Description: Human secreted CTLA4 , Fc fusion protein, also known as Cytotoxic T-lymphocyte-associated protein 4 and CD152. GenBank Accession No. NM_005214, a.a. 36-162 expressed in a HEK293 cell expression system. MW = 40.3 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human CD226 antigen also_x000D_known as DNAX accessory molecule 1_x000D_(DNAM-1), GenBank Accession No._x000D_NM_006566, a.a. 19-247 fused to Fc region_x000D_of human IgG1, expressed in a HEK293 cell_x000D_expression system. MW = 52.8 kDa_x000D_(monomer). This protein runs at a higher_x000D_MW by SDS-PAGE due to glycosylation.
Description: Secreted frizzled receptor (FZD1)-Fc fusion_x000D_protein and WNT receptor, also known as_x000D_FZD1, GenBank Accession No.BC_051271,_x000D_a.a. 73-253, expressed in a HEK293 cell_x000D_expression system. MW = 46.4 kDa_x000D_(monomer). This protein runs at a higher_x000D_apparent M.W. by SDS-PAGE due to_x000D_glycosylation.
Description: Secreted frizzled receptor (FZD4)-Fc fusion_x000D_protein and WNT receptor, also known as_x000D_FZD4, GenBank Accession No._x000D_BC_114527, a.a. 37-180, expressed in a_x000D_HEK293 cell expression system. MW = 42.9_x000D_kDa (monomer). This protein runs at a_x000D_higher apparent M.W. by SDS-PAGE due to_x000D_glycosylation.
Description: Secreted frizzled receptor (FZD7)-Fc fusion_x000D_protein and WNT receptor, also known as_x000D_FZD7, GenBank Accession No._x000D_BC_015915, a.a. 33-185, expressed in a_x000D_HEK293 cell expression system. MW = 43.3_x000D_kDa (monomer). This protein runs at a_x000D_higher apparent M.W. by SDS-PAGE due to_x000D_glycosylation.
Description: Secreted frizzled receptor (FZD10)-Fc_x000D_fusion protein and WNT receptor, also_x000D_known as FZD10, GenBank Accession No._x000D_BC_074997, a.a. 21-161, expressed in a_x000D_HEK293 cell expression system. MW = 42.7_x000D_kDa (monomer). This protein runs at a_x000D_higher apparent M.W. by SDS-PAGE due to_x000D_glycosylation.
Description: Human secreted B7-1-Fc fusion protein, also known as CD80. GenBank Accession No. NM_005191, a.a. 35-242 expressed in a HEK293 cell expression system. MW = 50.5 kDa (monomer). This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
Description: Human secreted B7-2, Fc fusion protein, also known as T-lymphocyte activation antigen CD86, B7.2, FUN-1, B70, BU63, and CD86. GenBank Accession No. NM_006889, a.a. 20-239 expressed in a HEK293 cell expression system. MW = 51.9 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human secreted B7-H5, Fc fusion protein, also known as Gi24, Dies1, VISTA, and SISP1, GenBank Accession No. NM_022153, a.a. 33-194 expressed in a HEK293 cell expression system. MW = 46.7 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
