Klonen van een HcCreb-gen en analyse van de effecten ervan op paarlemoerkleur en melaninesynthese in Hyriopsis cumingii
Creb (Cyclic AMP response ingredient bindend eiwit) is een nucleaire regulerende issue die transcriptie reguleert door autofosforylering. In melanocyten binden de overeenkomstige elementen van cAMP aan het Creb-eiwit voor autofosforylering en activeren ze MITF (Microphthalmia-associated transcription issue) . MITF stimuleert Tyrosine (tyr) om melanocyten te induceren om te differentiëren in eumelanine en pheomelanine. In deze studie werd een HcCreb-gen in Hyriopsis cumingii gekloond en de effecten ervan op de melaninesynthese en de paarlemoerkleur werden bestudeerd. HcCreb kwam tot expressie in zowel paarse als witte mosselen en er was een important verschil in expressie tussen adductoren (p<0,01) en mantelweefsel (p<0,05) .
Andere weefsels vertoonden geen significante verschillen (behalve voor kieuwweefsel), en in het algemeen was het niveau van mRNA-expressie hoger in paarse mosselen dan in witte mosselen. In zowel witte als paarse mosselen was de expressie in kieuwweefsel het hoogst, gevolgd door de mantel. Er werden sterke en specifieke mRNA-signalen gedetecteerd in de dorsale epitheelcellen van de palliale laag van de mantel, wat aangeeft dat HcCreb mogelijk betrokken is bij de vorming van parelmoer. Na behandeling met arbutine nam de expressie van HcCreb important af. Door de veranderingen in het melaninegehalte van de mantel verder te testen, werd gevonden dat het melaninegehalte na behandeling met arbutine important afnam in vergelijking met de controlegroep (p<0,05) . Er wordt gespeculeerd dat het HcCreb-gen een rol speelt in het proces van melaninesynthese en parelkleurvorming in H. cumingii.
Een Ab Initio meervoudige kloneringsmethode voor niet-adiabatische moleculaire dynamiek in opgewonden toestand in NWChem
De latest ontwikkelde ab initio a number of cloning (AIMC) -benadering op foundation van de multiconfigurationele Ehrenfest (MCE) -methode biedt een krachtige en nauwkeurige manier om de dynamiek van de aangeslagen toestand van moleculaire systemen te beschrijven. De AIMC-methode is een gecontroleerde benadering van niet-adiabatische dynamiek met een bijzondere kracht in de juiste beschrijving van decoherentie-effecten vanwege de vertakking van trillingsgolfpakketten bij een overweg. Hier rapporteren we een nieuwe implementatie van het AIMC-algoritme in het open supply NWChem computationele chemieprogramma.
Het raamwerk combineert lineaire-responstijdafhankelijke dichtheidsfunctionaaltheorie met Ehrenfest-gemiddelde-veldtheorie om de bewegingsvergelijkingen voor klassieke trajecten te bepalen . De multidimensionale golffunctie wordt ontleed in een superpositie van Gaussiaanse coherente toestanden geleid door Ehrenfest-trajecten (dwz MCE-benadering), die kunnen klonen met volledig kwantummechanische amplituden en fasen. Door gebruik te maken van een efficiënte op tijd gebaseerde niet-adiabatische koppelingsbenadering binnen de AIMC-methode, worden alle waarneembare waarden on-the-fly berekend in het niet-adiabatische moleculaire dynamicaproces.
Als representatief voorbeeld passen we onze implementatie toe om de ultrasnelle foto-geïnduceerde elektronische en vibrerende energieoverdracht in een pyridinemolecuul te bestuderen. De effecten van de kloonprocedure op elektronische en vibrationele coherentie, relaxatie en unidirectionele energieoverdracht worden besproken. Deze nieuwe AIMC-implementatie biedt een niet-adiabatisch moleculaire dynamica-raamwerk op hoog niveau voor het simuleren van foto-geëxciteerde dynamica in complexe moleculaire systemen en experimenteel relevante ultrasnelle spectroscopische sondes, zoals niet-lineaire coherente optische en röntgensignalen.
osteoporoseliga
Cold Fusion Cloning Kit with Competent Cells (50 rxns) International Sales Only
Description: The Quick PCR™ Plus Assembly Kit is used as a molecular cloning tool to assemble long DNA fragment from multiple smaller fragments, or to insert DNA into a plasmid in a single reaction. The main kit component is a ready-to-use mix of enzymes in reaction buffer at a 2-fold concentration. This kit also includes chemically competent E. coli cells (another version of the kit does not include competent cells, BPS Bioscience #78531).
Description: The Quick PCR™ Plus Assembly Kit is used as a molecular cloning tool to assemble long DNA fragment from multiple smaller fragments, or to insert DNA into a plasmid in a single reaction. The main kit component is a ready-to-use mix of enzymes in reaction buffer at a 2-fold concentration. This kit also includes chemically competent E. coli cells (another version of the kit does not include competent cells, BPS Bioscience #78531).
Fast and Efficient Mutagenesis Kit without Competent Cells
Description: ProClone™ Competent Cells are high-efficiency, chemically competent DH5α (E. coli) cells that are optimized for use with abm’s versatile range of expression vectors. Transformation efficiency greater than 1x10^6 cfu/µg can be achieved using abm’s expression vectors, which are typically 3 to 4-fold larger in size and contain more complex genetic elements than the standard pUC19 plasmid. Reduced recombination and highly transformation efficiency make ProClone™ Competent Cells the premier choice for both routine and challenging subcloning projects.
Description: BirA-transformed Competent E. coli cells are supplied as 10 x 50 µl vials._x000D_Strain BL21, a chemically competent E. coli B strain, contains an IPTG-inducible BirA_x000D_expression plasmid and constitutively-expressed streptomycin/spectinomycin resistance gene._x000D_These cells are compatible with most cloning vectors and are suitable for expression and_x000D_biotinylation of recombinant proteins using AviTag™ technology.
Description: Intact Genomics BL21 chemically competent cells are suitable for transformation and routine protein expression from non-T7 vectors.Product Includes:BL21 competent cellspUC19 control DNARecovery medium
Description: Intact Genomics ig™ 10B chemically competent cells (E. coli) are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. This derivative of DH10B provides the highest efficiency in the industry.Product Includes:ig™ 10B competent cellspUC19 control DNARecovery medium
Description: Intact Genomics ig™ 10B chemically competent cells (E. coli) are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. This derivative of DH10B provides the highest efficiency in the industry.Product Includes:ig™ 10B competent cellspUC19 control DNARecovery medium
Description: Intact Genomics ig™ 10B chemically competent cells (E. coli) are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. This derivative of DH10B provides the highest efficiency in the industry.Product Includes:ig™ 10B competent cellspUC19 control DNARecovery medium
Description: Intact Genomics ig™ 10B chemically competent cells (E. coli) are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. This derivative of DH10B provides the highest efficiency in the industry.Product Includes:ig™ 10B competent cellspUC19 control DNARecovery medium
Description: Intact Genomics JM109 chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning.
Description: Intact Genomics (ig®) HB101 chemically competent E. coli cells are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning. E. coli HB101 is a K12 x B hybrid strain, containing the recA13 mutation that minimizes recombination and helps insert stability. In addition, it carries the hsdS20(rB-mB-) restriction minus genotype which prevents cleavage of cloned DNA by endogenous restriction enzymes. HB101 strain does not support Alpha-complementation for blue/white screening
Description: Intact Genomics ig™ 10B chemically competent cells (E. coli) are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. This derivative of DH10B provides the highest efficiency in the industry.Product Includes:ig™ 10B competent cellspUC19 control DNARecovery medium
Description: Intact Genomics ig™ 10B chemically competent cells (E. coli) are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. This derivative of DH10B provides the highest efficiency in the industry.Product Includes:ig™ 10B competent cellspUC19 control DNARecovery medium
Description: Intact Genomics ig™ 10B chemically competent cells (E. coli) are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. This derivative of DH10B provides the highest efficiency in the industry.Product Includes:ig™ 10B competent cellspUC19 control DNARecovery medium
Description: Intact Genomics JM109 chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning.
Description: Intact Genomics (ig®) HB101 chemically competent E. coli cells are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning. E. coli HB101 is a K12 x B hybrid strain, containing the recA13 mutation that minimizes recombination and helps insert stability. In addition, it carries the hsdS20(rB-mB-) restriction minus genotype which prevents cleavage of cloned DNA by endogenous restriction enzymes. HB101 strain does not support Alpha-complementation for blue/white screening
Description: Intact Genomics (ig®) chemically competent MG1655 chemically competent cells are suitable for routine DNA transformation and other research purposes. MG1655 is the “wild type” K-12 strain with minimal genetic manipulation having only been cured of the temperate bacteriophage lambda and F plasmid by ultraviolet light and acridine orange
Description: Intact Genomics Chemically Competent Agrobacterium Combo Pack is perfect for scientists who require multiple agrobacterium competent cell strains for their research. The ElectroCompetent Agrobacterium Combo Pack contains 3 50µl aliquots of GV3101, AGL1, EHA105 and LBA4404 along with pCambia and our proprietary Agro Recovery Media.The GV3101 strain has a C58 chromosomal background with rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) with gentamicin resistance. The GV3101 Ti plasmid has the T-DNA region sequences deleted and transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of several dicots such as Arabidopsis thaliana, tobacco, potato, and monocots like corn.The AGL1 strain has a C58 chromosomal background that carries an insertion mutation in its recA recombination gene which stabilizes recombinant plasmids. It also carries rifampicin and carbenicilin resistance in its genome for selection. AGL1 contains the Ti plasmid pTiBO542 from which the T-DNA region sequences have been deleted. Transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of Arabidopsis thaliana as well as maize and other monocots.The LBA4404 strain is useful for transgenic operations of tomatoes, tobacco and other plants. LBA4404 contains a rifampicin resistance gene (rif). LBA4404 strain also contains a octoprine-type Ti plasmid pAL4404 without self-transport function, which contains the vir gene.The EHA105 strain is useful for transgenic operations of rice, tobacco and other plants. EHA105 contains a rifampicin resistance gene (rif). EHA105 strain also contains an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, which contains the vir gene.
Description: The AGL1 strain (A. tumefaciens) has a C58 chromosomal background that carries an insertion mutation in its recA recombination gene which stabilizes recombinant plasmids. It also carries rifampicin and carbenicilin resistance in its genome for selection. AGL1 contains the Ti plasmid pTiBO542 from which the T-DNA region sequences have been deleted. Transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of Arabidopsis thaliana as well as maize and other monocots.Product Includes:• AGL1 Chemically Competent cells• pCAMBIA control DNA• Recovery medium
Description: Intact Genomics chemically competent DL39 (DE3) E. coli cells are specific for transformation and protein expression in order to uniformly and specifically label :e.g. phenylalanine or leucine residues. DL39 (DE3) can also be used to reduce NMR cross-labeling via transaminase activity for valine, leucine, isoleucine, aspartate, phenylalanine, tyrosine and tryptophan residuesProduct Includes:DL39 (DE3) competent cellspUC19 control DNARecovery medium
Description: Intact Genomics chemically competent BL21(DE3) E. coli cells are suitable for transformation and routine protein expression.Product Includes:BL21 (DE3) competent cellspUC19 control DNARecovery medium
Description: Intact Genomics chemically competent BL21(DE3) E. coli cells are suitable for transformation and routine protein expression.Product Includes:BL21 (DE3) competent cellspUC19 control DNARecovery medium
Description: Intact Genomics chemically competent BL21(DE3) E. coli cells are suitable for transformation and routine protein expression.Product Includes:BL21 (DE3) competent cellspUC19 control DNARecovery medium
Description: The AGL1 strain (A. tumefaciens) has a C58 chromosomal background that carries an insertion mutation in its recA recombination gene which stabilizes recombinant plasmids. It also carries rifampicin and carbenicilin resistance in its genome for selection. AGL1 contains the Ti plasmid pTiBO542 from which the T-DNA region sequences have been deleted. Transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of Arabidopsis thaliana as well as maize and other monocots.Product Includes:• AGL1 Chemically Competent cells• pCAMBIA control DNA• Recovery medium
Description: C58C1 Chemically Competent Agrobacterium cells are optimized for the highest transformation efficiency and are useful for various applications. The chromosomal background of C58C1 is C58. C58 is cured of the Ti plasmid pTiC58 resulting in C58C1. C58C1 Competent cells may be useful for transgenic operations that involve Arabidopsis and other plants. This Agrobacterium strain is streptomycin and rifampicin resistant.Reagents Included:• C58C1 Chemically Competent Agrobacterium• DNA (pCAMBIA1391z, 500 pg/µl)• Recovery medium
Description: Intact Genomics JM109(DE3) chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. JM109(DE3) is useful for the high-level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter.
Description: Intact Genomics chemically competent BL21(DE3) E. coli cells are suitable for transformation and routine protein expression.Product Includes:BL21 (DE3) competent cellspUC19 control DNARecovery medium
Description: Intact Genomics (ig®) MSU440 Chemically Competent Agrobacterium cells are made from a specific strain of Rhizobium rhizogenes (formerly Agrobacterium rhizogenes), Agrobacterium rhizogenes(str R) MSU440 Ri (agropine type). Agrobacterium rhizogenes is a soil-borne gram-negative bacterium that can infect most dicotyledons, a few monocotyledons and some gymnosperms. MSU440 Chemically Competent Agrobacterium are optimized for the highest transformation efficiencies and are useful for transgenic operations of corn, tobacco, wormwood, tea tree and other plants. MSU440 Agrobacterium rhizogenes strain contains agrobacterium-type Ri plasmid and displays streptomycin resistance.
Description: GV3101 strain (A. tumefaciens) has a C58 chromosomal background with rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) with gentamicin resistance. The GV3101 Ti plasmid has the T-DNA region sequences deleted and transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of several dicots such as Arabidopsis thaliana, tobacco, potato, and monocots like corn.Product Includes:• GV3101 Chemically cells• pCAMBIA control DNA• Recovery medium
Description: Intact Genomics EHA105 Chemically Competent Agrobacterium cells are optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. The EHA105 strain is useful for transgenic operations of rice, tobacco and other plants. EHA105 contains a rifampicin resistance gene (rif). EHA105 strain also contains an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, which contains the vir gene.Product Includes:• EHA105 Chemically Competent cells• pCAMBIA control DNA• Recovery medium
Description: C58C1 Chemically Competent Agrobacterium cells are optimized for the highest transformation efficiency and are useful for various applications. The chromosomal background of C58C1 is C58. C58 is cured of the Ti plasmid pTiC58 resulting in C58C1. C58C1 Competent cells may be useful for transgenic operations that involve Arabidopsis and other plants. This Agrobacterium strain is streptomycin and rifampicin resistant.Reagents Included:• C58C1 Chemically Competent Agrobacterium• DNA (pCAMBIA1391z, 500 pg/µl)• Recovery medium
Description: Intact Genomics JM109(DE3) chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. JM109(DE3) is useful for the high-level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter.
Description: Intact Genomics (ig®) MSU440 Chemically Competent Agrobacterium cells are made from a specific strain of Rhizobium rhizogenes (formerly Agrobacterium rhizogenes), Agrobacterium rhizogenes(str R) MSU440 Ri (agropine type). Agrobacterium rhizogenes is a soil-borne gram-negative bacterium that can infect most dicotyledons, a few monocotyledons and some gymnosperms. MSU440 Chemically Competent Agrobacterium are optimized for the highest transformation efficiencies and are useful for transgenic operations of corn, tobacco, wormwood, tea tree and other plants. MSU440 Agrobacterium rhizogenes strain contains agrobacterium-type Ri plasmid and displays streptomycin resistance.
Description: GV3101 strain (A. tumefaciens) has a C58 chromosomal background with rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) with gentamicin resistance. The GV3101 Ti plasmid has the T-DNA region sequences deleted and transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of several dicots such as Arabidopsis thaliana, tobacco, potato, and monocots like corn.Product Includes:• GV3101 Chemically cells• pCAMBIA control DNA• Recovery medium
Description: Intact Genomics EHA105 Chemically Competent Agrobacterium cells are optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. The EHA105 strain is useful for transgenic operations of rice, tobacco and other plants. EHA105 contains a rifampicin resistance gene (rif). EHA105 strain also contains an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, which contains the vir gene.Product Includes:• EHA105 Chemically Competent cells• pCAMBIA control DNA• Recovery medium
Description: Intact Genomics LBA4404 Chemically Competent Agrobacterium cells are optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. The LBA4404 strain is useful for transgenic operations of tomatoes, tobacco and other plants. LBA4404 contains a rifampicin resistance gene (rif). LBA4404 strain also contains a octoprine-type Ti plasmid pAL4404 without self-transport function, which contains the vir gene.Product Includes:• LBA4404 Chemically Competent cells• pCAMBIA control DNA• Recovery medium
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Glucosiden in biodiesel
Biodiesels op foundation van plantaardige olie hebben een groot kwaliteitsprobleem vanwege de aanwezigheid van precipitaten gevormd door sterylglucosiden, die filters en injectoren van dieselmotoren verstoppen . Er is een efficiënte, schaalbare en kosteneffectieve methode ontwikkeld om sterylglucosiden te hydrolyseren met behulp van thermostabiele enzymen. Hier worden methoden gepresenteerd om enzymen met SGase- activiteit te ontdekken, tot expressie te brengen in recombinante micro-organismen en te produceren , evenals methoden om biodiesel met dergelijke enzymen te behandelen en om het gehalte aan sterylglucosiden in biodieselmonsters te meten.
Klonering , karakterisering en expressie van een gen dat codeert voor endo-1,4-β-xylanase van de schimmel Termitomyces clypeatus
Enzymatische afbraak van hemi-cellulosesubstraten heeft de laatste tijd veel industriële aandacht gekregen. Volledige enzymatische afbraak van complexe en weerbarstige hemicellulose vereist een enzymatische cocktail die voornamelijk bestaat uit endo-1,4-β-xylanase (xyl), β-xylosidase, arabinofuranosidase enz . Dit artikel rapporteert voor het eerst de identificatie, klonering, expressie en gedeeltelijke karakterisering van een krachtig endo-1,4- ,-xylanasegen (pxyl) van de paddenstoel Termitomyces clypeatus (TC) in E. coli en S. cerevisiae .
Het cDNA voor pxyl bleek 678 bp te zijn, wat op zijn beurt aanleiding geeft tot een voorlopereiwit (Pxyl) van 225 aminozuren lang wanneer het wordt gekloneerd in een prokaryotische expressievector. Om bovendien te karakteriseren, werd het cDNA ook tot expressie gebracht in S. cerevisiae. Bioinformatica-onderzoek voorspelde dat de Pxyl een leiderpeptide van 19 aminozuren bevat dat post-translationele modificaties mogelijk maakt, waaronder glycosylering, evenals de efficiënte secretie ervan in het medium. Van het recombinante eiwit is gevonden dat het een lid is van de GH11-familie met twee verre glutaminezuren als katalytische residuen. Dit rapport beschrijft nog een nieuwe en krachtige bron van xylanase voor commerciële exploitatie door de industrie in de toekomst.
Klonen , sequentieanalyse en weefselexpressie van paraoxonase van zijdeaapjes 1
Paraoxonase (PON) speelt een rol in het metabolisme van organofosfaatxenobiotica en geneesmiddelen. Ondanks het belang van zijdeaapjes voor onderzoek naar het metabolisme en de farmacokinetiek van geneesmiddelen, is paraoxonase van zijdeaapjes nog niet volledig gekarakteriseerd. Bijgevolg identificeerden we het PON1-gen in het genoom van de zijdeaapje door middel van sequentiehomologie-analyse. Marmoset PON1 cDNA met een open leesraam (1065 bp) werd met succes gekloneerd uit de lever van marmoset door middel van een reverse transcriptie-polymerase kettingreactie. De afgeleide aminozuursequentie (355 aminozuren) heeft ongeveer 93% identiteit met de menselijke ortholoog en bevat belangrijke aminozuurresiduen voor substraatbinding en calciumioncoördinatie.
Volgens een fylogenetische increase van PON1-aminozuursequenties, geconstrueerd met behulp van gegevens van zeven diersoorten, ligt zijdeaapje PON1 dichter bij menselijk PON1 dan bij de PON1-orthologen van proefdieren zoals varkens, konijnen, ratten en muizen. Marmoset PON1-mRNA werd voornamelijk tot expressie gebracht in de lever van de vijf onderzochte weefsels. Marmoset PON1-eiwit uitgescheiden in plasma werd gedetecteerd door immunoblotting. De paraoxon-hydrolyserende activiteit in plasma was hoger bij zijdeaapjes dan bij mensen . Op foundation van deze gegevens hebben we geconcludeerd dat zijdeaapje en humaan PON1 vergelijkbare kenmerken hebben met betrekking tot de genomische structuur, aminozuursequenties en weefseldistributie.
Een nieuwe pH en thermotolerante halofiele alfa-amylase van matige halofiele Nesterenkonia sp. stam F: genanalyse, moleculaire klonering , heterologe expressie en biochemische karakterisering
Een nieuwe pH en thermotolerante halofiele alfa-amylase van een matig halofiele bacterie, Nesterenkonia sp. stam F, werd gekloneerd en tot expressie gebracht in Escherichia coli . 16S rRNA-sequentie van de stam deelde 99,46% overeenkomsten met nauw verwante typesoorten. Ook deelde de genoomsequentie ANI-waarden van minder dan 92% en dDDH-waarden van minder dan 52% met de nauw verwante typesoorten. Bijgevolg wordt voorgesteld dat stam F een nieuwe soort vertegenwoordigt. Het AmyF-gen was 1390 bp lang en codeert voor een alfa-amylase van 463 aminozuurresiduen met een pI van 4,62. De afgeleide AmyF deelde een zeer lage sequentieovereenkomst (< 24%) met functioneel gekarakteriseerde recombinante halofiele alfa-amylasen.
Het recombinante alfa-amylase werd met succes gezuiverd uit Ni-NTA-kolommen met een molecuulmassa van ongeveer 52 KDa op natriumdodecylsulfaat-polyacrylamidegelelektroforese. Het enzym was actief over een breed temperatuurbereik (25-75°C) en pH (4-9) met een optimale activiteit bij respectievelijk 45°C en 7,5. Ook werd, hoewel het actief was bij verschillende concentraties NaCl en KCl (0-Four M), een toenemende activiteit van het enzym waargenomen bij toenemende concentratie van deze zouten. Lage concentraties Ca2 + -ionen hadden geen activerend impact, maar hoge concentraties van het ion (40-200 mM) versterkten de activiteit van AmyF.
De enzymactiviteit werd verhoogd door toenemende concentraties van Mg2 + , Zn2 + , Hg2 + en Fe3 + . Het werd echter alleen geremd bij zeer hoge concentraties van deze metaalionen. Cu2 + verminderde de amylase-activiteit niet en de hoogste activiteit werd waargenomen bij 100 mM van het ion. Deze eigenschappen duiden op brede potentiële toepassingen van dit recombinante enzym in zetmeelverwerkende industrieën. Dit is de eerste isolatie , klonering en karakterisering van een gen dat codeert voor alfa-amylase van het geslacht Nesternkonia.
Cold Fusion Cloning Kit with Competent Cells (50 rxns) International Sales Only
Description: The Quick PCR™ Plus Assembly Kit is used as a molecular cloning tool to assemble long DNA fragment from multiple smaller fragments, or to insert DNA into a plasmid in a single reaction. The main kit component is a ready-to-use mix of enzymes in reaction buffer at a 2-fold concentration. This kit also includes chemically competent E. coli cells (another version of the kit does not include competent cells, BPS Bioscience #78531).
Description: The Quick PCR™ Plus Assembly Kit is used as a molecular cloning tool to assemble long DNA fragment from multiple smaller fragments, or to insert DNA into a plasmid in a single reaction. The main kit component is a ready-to-use mix of enzymes in reaction buffer at a 2-fold concentration. This kit also includes chemically competent E. coli cells (another version of the kit does not include competent cells, BPS Bioscience #78531).
Fast and Efficient Mutagenesis Kit without Competent Cells
Description: ProClone™ Competent Cells are high-efficiency, chemically competent DH5α (E. coli) cells that are optimized for use with abm’s versatile range of expression vectors. Transformation efficiency greater than 1x10^6 cfu/µg can be achieved using abm’s expression vectors, which are typically 3 to 4-fold larger in size and contain more complex genetic elements than the standard pUC19 plasmid. Reduced recombination and highly transformation efficiency make ProClone™ Competent Cells the premier choice for both routine and challenging subcloning projects.
Description: BirA-transformed Competent E. coli cells are supplied as 10 x 50 µl vials._x000D_Strain BL21, a chemically competent E. coli B strain, contains an IPTG-inducible BirA_x000D_expression plasmid and constitutively-expressed streptomycin/spectinomycin resistance gene._x000D_These cells are compatible with most cloning vectors and are suitable for expression and_x000D_biotinylation of recombinant proteins using AviTag™ technology.
Description: Intact Genomics BL21 chemically competent cells are suitable for transformation and routine protein expression from non-T7 vectors.Product Includes:BL21 competent cellspUC19 control DNARecovery medium
Description: Intact Genomics ig™ 10B chemically competent cells (E. coli) are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. This derivative of DH10B provides the highest efficiency in the industry.Product Includes:ig™ 10B competent cellspUC19 control DNARecovery medium
Description: Intact Genomics ig™ 10B chemically competent cells (E. coli) are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. This derivative of DH10B provides the highest efficiency in the industry.Product Includes:ig™ 10B competent cellspUC19 control DNARecovery medium
Description: Intact Genomics ig™ 10B chemically competent cells (E. coli) are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. This derivative of DH10B provides the highest efficiency in the industry.Product Includes:ig™ 10B competent cellspUC19 control DNARecovery medium
Description: Intact Genomics ig™ 10B chemically competent cells (E. coli) are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. This derivative of DH10B provides the highest efficiency in the industry.Product Includes:ig™ 10B competent cellspUC19 control DNARecovery medium
Description: Intact Genomics JM109 chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning.
Description: Intact Genomics (ig®) HB101 chemically competent E. coli cells are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning. E. coli HB101 is a K12 x B hybrid strain, containing the recA13 mutation that minimizes recombination and helps insert stability. In addition, it carries the hsdS20(rB-mB-) restriction minus genotype which prevents cleavage of cloned DNA by endogenous restriction enzymes. HB101 strain does not support Alpha-complementation for blue/white screening
Description: Intact Genomics ig™ 10B chemically competent cells (E. coli) are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. This derivative of DH10B provides the highest efficiency in the industry.Product Includes:ig™ 10B competent cellspUC19 control DNARecovery medium
Description: Intact Genomics ig™ 10B chemically competent cells (E. coli) are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. This derivative of DH10B provides the highest efficiency in the industry.Product Includes:ig™ 10B competent cellspUC19 control DNARecovery medium
Description: Intact Genomics ig™ 10B chemically competent cells (E. coli) are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. This derivative of DH10B provides the highest efficiency in the industry.Product Includes:ig™ 10B competent cellspUC19 control DNARecovery medium
Description: Intact Genomics JM109 chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning.
Description: Intact Genomics (ig®) HB101 chemically competent E. coli cells are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning. E. coli HB101 is a K12 x B hybrid strain, containing the recA13 mutation that minimizes recombination and helps insert stability. In addition, it carries the hsdS20(rB-mB-) restriction minus genotype which prevents cleavage of cloned DNA by endogenous restriction enzymes. HB101 strain does not support Alpha-complementation for blue/white screening
Description: Intact Genomics (ig®) chemically competent MG1655 chemically competent cells are suitable for routine DNA transformation and other research purposes. MG1655 is the “wild type” K-12 strain with minimal genetic manipulation having only been cured of the temperate bacteriophage lambda and F plasmid by ultraviolet light and acridine orange
Description: Intact Genomics Chemically Competent Agrobacterium Combo Pack is perfect for scientists who require multiple agrobacterium competent cell strains for their research. The ElectroCompetent Agrobacterium Combo Pack contains 3 50µl aliquots of GV3101, AGL1, EHA105 and LBA4404 along with pCambia and our proprietary Agro Recovery Media.The GV3101 strain has a C58 chromosomal background with rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) with gentamicin resistance. The GV3101 Ti plasmid has the T-DNA region sequences deleted and transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of several dicots such as Arabidopsis thaliana, tobacco, potato, and monocots like corn.The AGL1 strain has a C58 chromosomal background that carries an insertion mutation in its recA recombination gene which stabilizes recombinant plasmids. It also carries rifampicin and carbenicilin resistance in its genome for selection. AGL1 contains the Ti plasmid pTiBO542 from which the T-DNA region sequences have been deleted. Transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of Arabidopsis thaliana as well as maize and other monocots.The LBA4404 strain is useful for transgenic operations of tomatoes, tobacco and other plants. LBA4404 contains a rifampicin resistance gene (rif). LBA4404 strain also contains a octoprine-type Ti plasmid pAL4404 without self-transport function, which contains the vir gene.The EHA105 strain is useful for transgenic operations of rice, tobacco and other plants. EHA105 contains a rifampicin resistance gene (rif). EHA105 strain also contains an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, which contains the vir gene.
Description: The AGL1 strain (A. tumefaciens) has a C58 chromosomal background that carries an insertion mutation in its recA recombination gene which stabilizes recombinant plasmids. It also carries rifampicin and carbenicilin resistance in its genome for selection. AGL1 contains the Ti plasmid pTiBO542 from which the T-DNA region sequences have been deleted. Transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of Arabidopsis thaliana as well as maize and other monocots.Product Includes:• AGL1 Chemically Competent cells• pCAMBIA control DNA• Recovery medium
Description: Intact Genomics chemically competent DL39 (DE3) E. coli cells are specific for transformation and protein expression in order to uniformly and specifically label :e.g. phenylalanine or leucine residues. DL39 (DE3) can also be used to reduce NMR cross-labeling via transaminase activity for valine, leucine, isoleucine, aspartate, phenylalanine, tyrosine and tryptophan residuesProduct Includes:DL39 (DE3) competent cellspUC19 control DNARecovery medium
Description: Intact Genomics chemically competent BL21(DE3) E. coli cells are suitable for transformation and routine protein expression.Product Includes:BL21 (DE3) competent cellspUC19 control DNARecovery medium
Description: Intact Genomics chemically competent BL21(DE3) E. coli cells are suitable for transformation and routine protein expression.Product Includes:BL21 (DE3) competent cellspUC19 control DNARecovery medium
Description: Intact Genomics chemically competent BL21(DE3) E. coli cells are suitable for transformation and routine protein expression.Product Includes:BL21 (DE3) competent cellspUC19 control DNARecovery medium
Description: The AGL1 strain (A. tumefaciens) has a C58 chromosomal background that carries an insertion mutation in its recA recombination gene which stabilizes recombinant plasmids. It also carries rifampicin and carbenicilin resistance in its genome for selection. AGL1 contains the Ti plasmid pTiBO542 from which the T-DNA region sequences have been deleted. Transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of Arabidopsis thaliana as well as maize and other monocots.Product Includes:• AGL1 Chemically Competent cells• pCAMBIA control DNA• Recovery medium
Description: C58C1 Chemically Competent Agrobacterium cells are optimized for the highest transformation efficiency and are useful for various applications. The chromosomal background of C58C1 is C58. C58 is cured of the Ti plasmid pTiC58 resulting in C58C1. C58C1 Competent cells may be useful for transgenic operations that involve Arabidopsis and other plants. This Agrobacterium strain is streptomycin and rifampicin resistant.Reagents Included:• C58C1 Chemically Competent Agrobacterium• DNA (pCAMBIA1391z, 500 pg/µl)• Recovery medium
Description: Intact Genomics JM109(DE3) chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. JM109(DE3) is useful for the high-level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter.
Description: Intact Genomics chemically competent BL21(DE3) E. coli cells are suitable for transformation and routine protein expression.Product Includes:BL21 (DE3) competent cellspUC19 control DNARecovery medium
